Antimycin A treatment decreases respiratory internal rotenone-insensitive NADH oxidation capacity in potato leaves.
Antimycin A treatment decreases respiratory internal rotenone-insensitive NADH oxidation capacity in potato leaves.
复制标题
DOI：
10.1186/1471-2229-4-8
复制
发表时间：
2004-05-12
影响因子：
5.3
通讯作者：
Rasmusson AG
中科院分区：
文献类型：
--
作者：
Geisler DA;Johansson FI;Svensson AS;Rasmusson AG
植物呼吸链含有几种能量耗散酶，这些酶是II型NAD(P)H脱氢酶和交替氧化酶，在哺乳动物中不存在。II型NAD(P)H脱氢酶的生理功能在很大程度上还不清楚，对它们对应激的反应也知之甚少。本研究对马铃薯植株(Solanum tuberosum L.，cv.)Desiree)，喷洒细胞色素途径的抑制剂抗霉素A。然后分析了叶片线粒体中NAD(P)H脱氢酶(EC 1.6.5.3)和交替氧化酶的活性。我们报道了抗霉素A处理的叶片线粒体内鱼藤酮不敏感的NADH脱氢酶能力的特殊降低。外源NADPH脱氢酶和交替氧化酶能力仍未受到处理的影响。Western blotting显示，NAD(P)H脱氢酶同系物NDA1和NDB1的蛋白质丰度没有变化，也没有改变复合体I的两个亚基的蛋白质丰度，交替氧化酶最多只有轻微的增加。Nda1的转录水平，以及来自之前未被研究的密切相关的马铃薯同源物的表达序列标签，在处理过程中保持不变。与每日节律调节的NDA1相比，新的同源物在研究的时间内表现出稳定的转录水平。马铃薯叶片经抗霉素A处理后，体内鱼藤酮不敏感的NADH氧化减少。然而，这种减少并不是由于已知的NDA基因表达的变化。如果细胞色素和替代途径的总体能力受到限制，NADH脱氢酶能力降低的一个后果可能是呼吸链还原水平的稳定。
The plant respiratory chain contains several energy-dissipating enzymes, these being type II NAD(P)H dehydrogenases and the alternative oxidase, not present in mammals. The physiological functions of type II NAD(P)H dehydrogenases are largely unclear and little is known about their responses to stress. In this investigation, potato plants (Solanum tuberosum L., cv. Desiree) were sprayed with antimycin A, an inhibitor of the cytochrome pathway. Enzyme capacities of NAD(P)H dehydrogenases (EC 1.6.5.3) and the alternative oxidase were then analysed in isolated leaf mitochondria. We report a specific decrease in internal rotenone-insensitive NADH dehydrogenase capacity in mitochondria from antimycin A-treated leaves. External NADPH dehydrogenase and alternative oxidase capacities remained unaffected by the treatment. Western blotting revealed no change in protein abundance for two characterised NAD(P)H dehydrogenase homologues, NDA1 and NDB1, nor for two subunits of complex I. The alternative oxidase was at most only slightly increased. Transcript levels of nda1, as well as an expressed sequence tag derived from a previously uninvestigated closely related potato homologue, remained unchanged by the treatment. As compared to the daily rhythm-regulated nda1, the novel homologue displayed steady transcript levels over the time investigated. The internal rotenone-insensitive NADH oxidation decreases after antimycin A treatment of potato leaves. However, the decrease is not due to changes in expression of known nda genes. One consequence of the lower NADH dehydrogenase capacity may be a stabilisation of the respiratory chain reduction level, should the overall capacity of the cytochrome and the alternative pathway be restricted.
登录
查看更多内容
影响因子：
7.4
作者：
Raha, S;McEachern, GE;Robinson, BH
通讯作者：
Robinson, BH
影响因子：
4.3
作者：
Rasmusson, AG;Heiser, V;Grohmann, L
通讯作者：
Grohmann, L
影响因子：
7.4
作者：
RASMUSSON, AG;MOLLER, IM
通讯作者：
MOLLER, IM
影响因子：
1.1
作者：
HUANG, XQ;MILLER, W
通讯作者：
MILLER, W
DOI：
10.1073/pnas.96.14.8271
发表时间：
1999-07-06
影响因子：
11.1
作者：
Maxwell, DP;Wang, Y;McIntosh, L
通讯作者：
McIntosh, L