Fiber optic probe for polarized reflectance spectroscopy in vivo: design and performance.
Fiber optic probe for polarized reflectance spectroscopy in vivo: design and performance.
复制标题
用于体内偏振反射光谱的光纤探头：设计和性能。
DOI：
--
复制
发表时间：
2002
影响因子：
3.5
通讯作者：
K. Sokolov
中科院分区：
文献类型：
--
作者：
A. Myakov;Linda T. Nieman;Lorenz Wicky;U. Utzinger;R. Richards;K. Sokolov
本文介绍了一种用于活体弹性光散射光谱的偏振激发和偏振敏感检测的光纤探头的设计和制作。光纤探头的性能是使用放置在扩散散射衬底上的聚苯乙烯球体的悬浮液来评估的，它表明，在存在高度扩散散射背景的情况下，可以使用散射体的前向和后向Mie散射分量的线性组合来提取散射体的尺寸相关特征。随后，对核的平均直径、大小分布和相对折射率等广泛的诊断相关参数进行了Mie理论计算，以了解如何使用纤维探头的偏振反射比测量来提取关于上皮组织的形态信息。最后，论证了基于光纤的偏振敏感光散射光谱用于活体测量的可行性。
We present the design and construction of a fiber optic probe for elastic light scattering spectroscopy in vivo with polarized excitation and polarization sensitive detection. The performance of the fiber probe is evaluated using a suspension of polystyrene spheres placed atop a diffusely scattering substrate, and it demonstrates that the size-dependent characteristics of the scatterers can be extracted in the presence of a highly diffusely scattering background using a linear combination of forward and backward Mie scattering components of the scatterers. Subsequently, Mie theory calculations are performed over a broad range of diagnostically relevant parameters of nuclei-mean diameter, size distribution, and relative refractive index-to understand how the polarized reflectance measurements with the fiber probe can be used to extract morphological information about epithelial tissue. Finally, the feasibility of in vivo measurements with the fiber optic based polarization sensitive light scattering spectroscopy is demonstrated.
影响因子：
3.5
作者：
Sokolov, K;Galvan, J;Richards-Kortum, R
通讯作者：
Richards-Kortum, R