Dry season prevalence of Plasmodium falciparum in asymptomatic gambian children, with a comparative evaluation of diagnostic methods.
Dry season prevalence of Plasmodium falciparum in asymptomatic gambian children, with a comparative evaluation of diagnostic methods.
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DOI：
10.1186/s12936-022-04184-9
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发表时间：
2022-06-07
期刊：
影响因子：
3
通讯作者：
Riley, Eleanor M.
中科院分区：
文献类型：
--
作者：
Mooney, Jason P.;DonVito, Sophia M.;Jahateh, Maimuna;Bittaye, Haddy;Bottomley, Christian;D'Alessandro, Umberto;Riley, Eleanor M.
恶性疟原虫的亚临床感染仍然非常普遍，但诊断这些通常是低密度感染仍然是一个挑战。感染可以是亚专利的，低于常规厚膜显微镜和快速诊断测试（RDT）的检测极限。在本研究中，在2017/2018年旱季开始时，冈比亚上游河流地区的学龄儿童中亚临床恶性疟原虫感染的流行率被表征，目的是比较不同诊断工具的效用。对冈比亚河南岸29个村庄的儿童（中位年龄为10岁）进行了横断面调查，采用匹配显微镜、快速诊断试验（RDT，检测富组氨酸蛋白2）和聚合酶链反应（PCR，针对18S rRNA或var基因酸性末端序列）来确定专利感染和亚专利感染的患病率，并比较不同诊断方法的性能。var基因酸性末端序列（varATS） qpcr检测感染率为10.2%(141/1381)，中位密度为3.12只/µL。疟疾流行率在整个区域具有高度异质性，不同村庄群的流行率从< 1%到~ 40%不等。与varATS相比，18S rRNA PCR检测到的低密度感染较少，检测灵敏度为50%，特异性为98.8%。该队列的寄生虫流行率镜检为2.9%，RDT为1.5%。与varATS qPCR相比，显微镜和RDT的灵敏度分别为11.5%和9.2%，尽管这两种方法都具有很高的特异性（约为98%）。三种检测（varATS qPCR、RDT和显微镜检测）均呈阳性的样品的寄生虫密度（中位数= 1705只/µL）明显高于仅通过varATS qPCR检测阳性的样品（中位数= 2.4只/µL）。大多数学龄儿童亚临床疟疾感染的寄生虫密度极低，只能通过超灵敏PCR分析检测到。了解这些低密度感染的持续时间、它们的生理影响以及它们对寄生虫持续传播的贡献，对于为疟疾消除战略提供信息是必要的。在线版本包含补充材料，可在10.1186/s12936-022-04184-9获得。
Subclinical infection with Plasmodium falciparum remains highly prevalent, yet diagnosing these often low-density infections remains a challenge. Infections can be subpatent, falling below the limit of detection for conventional thick-film microscopy and rapid diagnostic testing (RDT). In this study, the prevalence of subclinical P. falciparum infections in school-aged children was characterised at the start of the dry season in the Upper River Region of The Gambia in 2017/2018, with a goal to also compare the utility of different diagnostic tools. In a cross-sectional survey of children living in 29 villages on the south bank of the Gambia river (median age of 10 years), matched microscopy, rapid diagnostic test (RDT, detecting histidine-rich protein 2) and polymerase chain reaction (PCR, targeting either 18S rRNA or var gene acidic terminal sequence) were used to determine the prevalence of patent and subpatent infections and to compare the performance of the different diagnostic methods. The prevalence of var gene acidic terminal sequence (varATS) qPCR-detectable infections was 10.2% (141/1381) with a median density of 3.12 parasites/µL. Malaria prevalence was highly heterogeneous across the region, ranging from < 1% to ~ 40% prevalence in different village clusters. Compared to varATS, 18S rRNA PCR detected fewer low-density infections, with an assay sensitivity of 50% and specificity of 98.8%. Parasite prevalence in the cohort was 2.9% by microscopy and 1.5% by RDT. Compared to varATS qPCR, microscopy and RDT had sensitivities of 11.5% and 9.2%, respectively, although both methods were highly specific (> 98%). Samples that were positive by all three tests (varATS qPCR, RDT and microscopy) had significantly higher parasite densities (median = 1705 parasites/µL) than samples that were positive by varATS qPCR only (median = 2.4 parasites/µL). The majority of subclinical malaria infections in school-aged children were of extremely low parasite density and detectable only by ultra-sensitive PCR analysis. Understanding the duration of these low density infections, their physiological impact and their contribution to sustained parasite transmission is necessary to inform malaria elimination strategies. The online version contains supplementary material available at 10.1186/s12936-022-04184-9.
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影响因子：
15.8
作者：
Chen I;Clarke SE;Gosling R;Hamainza B;Killeen G;Magill A;O'Meara W;Price RN;Riley EM
通讯作者：
Riley EM
DOI：
10.1016/s1473-3099(21)00072-4
发表时间：
2021-11
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Andolina C;Rek JC;Briggs J;Okoth J;Musiime A;Ramjith J;Teyssier N;Conrad M;Nankabirwa JI;Lanke K;Rodriguez-Barraquer I;Meerstein-Kessel L;Arinaitwe E;Olwoch P;Rosenthal PJ;Kamya MR;Dorsey G;Greenhouse B;Drakeley C;Staedke SG;Bousema T
通讯作者：
Bousema T
影响因子：
3
作者：
Manjurano A;Okell L;Lukindo T;Reyburn H;Olomi R;Roper C;Clark TG;Joseph S;Riley EM;Drakeley C
通讯作者：
Drakeley C
影响因子：
17.1
作者：
Joice R;Nilsson SK;Montgomery J;Dankwa S;Egan E;Morahan B;Seydel KB;Bertuccini L;Alano P;Williamson KC;Duraisingh MT;Taylor TE;Milner DA;Marti M
通讯作者：
Marti M
影响因子：
5.2
作者：
Frimpong A;Amponsah J;Adjokatseh AS;Agyemang D;Bentum-Ennin L;Ofori EA;Kyei-Baafour E;Akyea-Mensah K;Adu B;Mensah GI;Amoah LE;Kusi KA
通讯作者：
Kusi KA