Factors affecting the accuracy and reliability of the measurement of anti-Müllerian hormone concentration in the clinic.
Factors affecting the accuracy and reliability of the measurement of anti-Müllerian hormone concentration in the clinic.
复制标题
DOI：
10.1177/03000605211016161
复制
发表时间：
2021-05
期刊：
影响因子：
--
通讯作者：
Hu R
中科院分区：
文献类型：
--
作者：
Fu YX;Wang H;Hu T;Wang FM;Hu R
我们的目的是确定影响血清抗苗勒氏管激素 (AMH) 浓度测量的因素。我们收集了2018年5月至9月期间的血清样本，并比较了静脉穿刺、储存时间、储存温度、反应微板位置以及口服避孕药和促性腺激素释放激素（GnRH）的使用等条件对通过ELISA测量的AMH浓度的影响。 AMH 浓度不受食物摄入量影响，但受溶血影响。 ELISA 微量滴定板边缘的样品中的该值也高得多。室温培养1天、4℃培养3天、-20℃培养1个月和-40℃培养4个月后，AMH浓度增加，但-80℃保存9个月时，AMH浓度没有变化。 GnRH 激动剂治疗后患者的 AMH 浓度较高，但不受口服避孕药的影响。 AMH 测量前无需禁食。将血清样本放置在微量滴定板边缘会影响 AMH ELISA 的结果。如果不能立即检测血清样本，最好将其保存在-80°C。基础 AMH 浓度不能作为 GnRH 激动剂治疗后卵巢储备功能的衡量标准。
We aimed to identify the factors that influence serum anti-Müllerian hormone (AMH) concentration measurements. We collected serum samples between May and September 2018 and compared the effect on AMH concentration measured by ELISA of conditions including venepuncture, storage time, storage temperature, locations of the reaction microplate, and the use of the oral contraceptive pill and gonadotrophin-releasing hormone (GnRH). AMH concentration was not affected by food intake but was affected by haemolysis. It was also much higher in samples on the edge of the ELISA microtitre plate. AMH concentration increased after incubation at room temperature for 1 day, 4°C for 3 days, −20°C for 1 month and −40°C for 4 months, but no change occurred during storage at −80°C for 9 months. AMH concentration was high in patients following GnRH agonist treatment but was not affected by oral contraceptives. No fasting is required prior to AMH measurement. Placement of serum samples on the edge of microtitre plates affects the results of the AMH ELISA. If serum samples cannot be assayed immediately, it is best to store them at −80°C. Basal AMH concentration cannot be used as a measure of ovarian reserve after GnRH agonist treatment.
登录
查看更多内容
影响因子：
6.7
作者：
Seifer, David B.;Baker, Valerie L.;Leader, Benjamin
通讯作者：
Leader, Benjamin
影响因子：
5.8
作者：
Henry, N. Lynn;Xia, Rong;Hayes, Daniel F.
通讯作者：
Hayes, Daniel F.
DOI：
10.1016/j.ejogrb.2019.06.024
发表时间：
2019-09-01
影响因子：
2.6
作者：
Dong, Zhenzhu;An, Jian;Sun, Pengming
通讯作者：
Sun, Pengming
影响因子：
6.1
作者：
Fanchin, R;Taieb, J;Bouyer, J
通讯作者：
Bouyer, J
影响因子：
5.8
作者：
Dolleman, M.;Verschuren, W. M. M.;van der Schouw, Y. T.
通讯作者：
van der Schouw, Y. T.