Preparation of nucleotide advanced glycation endproducts--imidazopurinone adducts formed by glycation of deoxyguanosine with glyoxal and methylglyoxal.
Preparation of nucleotide advanced glycation endproducts--imidazopurinone adducts formed by glycation of deoxyguanosine with glyoxal and methylglyoxal.
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DOI：
10.1196/annals.1433.037
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发表时间：
2008-04
影响因子：
5.2
通讯作者：
Thornalley PJ
中科院分区：
文献类型：
--
作者：
Fleming T;Rabbani N;Thornalley PJ
建立了生理条件下乙二醛和甲基乙二醛与脱氧鸟苷反应形成的核苷酸晚期糖基化终产物的分析方法。为此，制备了咪唑嘌呤酮衍生物3-（2′-脱氧核糖基）-6,7-二氢-6,7-二羟基咪唑[2,3-b]嘌呤-9(8)1 （dG-G）和3-（2′-脱氧核糖基）-6,7-二氢-6,7-二羟基-6-甲基咪唑-[2,3-b]嘌呤-9(8)1 （dG-MG）。制备了真正的标准和稳定的同位素取代标准加合物，并采用液相色谱-串联质谱法和优化的DNA提取和核酸酶酶切程序建立了同位素稀释分析方法。对培养的人细胞和单核白细胞DNA中dG-G、dG-MG和氧化标记物8-羟基脱氧鸟苷的分析表明，核苷酸晚期糖基化终产物是人细胞DNA损伤的主要标志。
An analytical procedure was developed for nucleotide advanced glycation endproducts formed by the reaction of glyoxal and methylglyoxal with deoxyguanosine under physiological conditions. For this, the imidazopurinone derivatives, 3-(2′-deoxyribosyl)-6,7-dihydro-6,7-dihydroxyimidazo[2,3-b]purin-9(8)one (dG-G) and 3-(2′-deoxyribosyl)-6,7-dihydro-6,7-dihydroxy-6-methylimidazo-[2,3-b]purine-9(8)one (dG-MG), were prepared. Authentic standard and stable isotope-substituted standard adducts were prepared and an isotopic dilution analysis assay methodology was developed using liquid chromatography with tandem mass spectrometry and optimized DNA extraction and nuclease digestion procedures. Analysis of dG-G, dG-MG, and the oxidative marker 8-hydroxydeoxyguanosine in the DNA of cultured human cells and mononuclear leukocytes showed that nucleotide advanced glycation endproducts are major markers of DNA damage in human cells.
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影响因子：
64.8
作者：
Friedberg, EC
通讯作者：
Friedberg, EC
影响因子：
3.9
作者：
Thornalley, PJ
通讯作者：
Thornalley, PJ
影响因子：
4.1
作者：
Frischmann, M;Bidmon, C;Pischetsrieder, M
通讯作者：
Pischetsrieder, M
影响因子：
4.7
作者：
VACA, CE;FANG, JL;HOU, SM
通讯作者：
HOU, SM