Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring.
Microneedle Aptamer-Based Sensors for Continuous, Real-Time Therapeutic Drug Monitoring.
复制标题
基于微针适体的传感器，用于连续实时治疗药物监测。
DOI：
10.1021/acs.analchem.2c00829
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发表时间：
2022-06-14
影响因子：
7.4
通讯作者：
Arroyo-Curras, Netzahualcoyotl
中科院分区：
文献类型：
--
作者：
Wu, Yao;Tehrani, Farshad;Teymourian, Hazhir;Mack, John;Shaver, Alexander;Reynoso, Maria;Kavner, Jonathan;Huang, Nickey;Furmidge, Allison;Duvvuri, Andres;Nie, Yuhang;Laffel, Lori M.;Doyle, Francis J.;Patti, Mary-Elizabeth;Dassau, Eyal;Wang, Joseph;Arroyo-Curras, Netzahualcoyotl
连续监测体内特定分子浓度的能力是生物医学研究长期追求的目标。为此，间质液（ISF）被认为是理想的目标生物液，因为其成分可以快速与全身血液平衡，从而可以评估反映全身生理学的分子浓度。过去，ISF 的连续监测是通过微针传感器阵列实现的。然而，基准微针传感器只能检测经历氧化还原反应的分子，这限制了感测非氧化还原活性代谢物、生物标志物和治疗药物的能力。为了克服这一障碍，我们在这里通过展示首次使用微针支持的电化学适配体（E-AB）传感器来扩展这些设备的范围。该平台实现了基于亲和力相互作用的分子识别，极大地扩展了可感知分子的范围。我们报告了微针 E-AB 传感器阵列的制造以及一种将其再生以供多种用途的方法。此外，我们还演示了使用这些传感器在体外流动系统中使用单和多重微针阵列配置进行连续分子测量。正如我们在啮齿类动物的 ISF 中进行的首次 E-AB 测量所证明的那样，该平台可以转化为体内测量。这项工作报告的令人鼓舞的结果应作为未来将微针 E-AB 传感器阵列转化为临床前动物模型生物医学研究的基础。
The ability to continuously monitor the concentration of specific molecules in the body is a long-sought goal of biomedical research. For this purpose, interstitial fluid (ISF) was proposed as the ideal target biofluid because its composition can rapidly equilibrate with that of systemic blood, allowing the assessment of molecular concentrations that reflect full-body physiology. In the past, continuous monitoring in ISF was enabled by microneedle sensor arrays. Yet, benchmark microneedle sensors can only detect molecules that undergo redox reactions, which limits the ability to sense metabolites, biomarkers, and therapeutics that are not redox-active. To overcome this barrier, here, we expand the scope of these devices by demonstrating the first use of microneedle-supported electrochemical, aptamer-based (E-AB) sensors. This platform achieves molecular recognition based on affinity interactions, vastly expanding the scope of molecules that can be sensed. We report the fabrication of microneedle E-AB sensor arrays and a method to regenerate them for multiple uses. In addition, we demonstrate continuous molecular measurements using these sensors in flow systems in vitro using single and multiplexed microneedle array configurations. Translation of the platform to in vivo measurements is possible as we demonstrate with a first E-AB measurement in the ISF of a rodent. The encouraging results reported in this work should serve as the basis for future translation of microneedle E-AB sensor arrays to biomedical research in preclinical animal models.
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DOI：
10.1007/978-1-0716-1803-5_25
发表时间：
2022-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Dauphin-Ducharme, Philippe;Ploense, Kyle L;Plaxco, Kevin W
通讯作者：
Plaxco, Kevin W
影响因子：
11.8
作者：
Dilworth, Thomas J.;Schulz, Lucas T.;Rose, Warren E.
通讯作者：
Rose, Warren E.
影响因子：
50.5
作者：
Oakman, C.;Francis, P. A.;Di Leo, A.
通讯作者：
Di Leo, A.
影响因子：
--
作者：
Arroyo-Curras, Netzahualcoyotl;Ortega, Gabriel;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
影响因子：
8.4
作者：
Idili, Andrea;Arroyo-Curras, Netzahualcoyotl;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.