The cell biology of peritrichous flagella in Bacillus subtilis.
The cell biology of peritrichous flagella in Bacillus subtilis.
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DOI：
10.1111/mmi.12103
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发表时间：
2013-01
影响因子：
3.6
通讯作者：
Kearns DB
中科院分区：
文献类型：
--
作者：
Guttenplan SB;Shaw S;Kearns DB
Bacterial flagella are highly conserved molecular machines that have been extensively studied for assembly, function, and gene regulation. Less studied is how and why bacteria differ based on the number and arrangement of the flagella they synthesize. Here we explore the cell biology of peritrichous flagella in the model bacterium Bacillus subtilis by fluorescently labeling flagellar basal bodies, hooks, and filaments. We find that the average B. subtilis cell assembles approximately 26 flagellar basal bodies and we show that basal body number is controlled by the protein of unknown function SwrA. Basal bodies are assembled rapidly (< 5 minutes) but the assembly of flagella capable of supporting motility (> 40 minutes) is rate limited by filament polymerization. We find that basal bodies are not positioned randomly on the cell surface. Rather, basal bodies occupy a grid-like pattern organized symmetrically around the midcell and that flagella are discouraged at the poles. Basal body position is genetically determined by FlhF and FlhG homologs to control spatial patterning differently from what is seen in bacteria with polar flagella. Finally, spatial control of flagella in B. subtilis seems more relevant to the inheritance of flagella in individual cells than the motile behavior of populations.
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影响因子：
3.5
作者：
GueroutFleury, AM;Frandsen, N;Stragier, P
通讯作者：
Stragier, P
影响因子：
3.2
作者：
Correa, NE;Peng, F;Klose, KE
通讯作者：
Klose, KE
影响因子：
56.9
作者：
Blair, Kris M.;Turner, Linda;Kearns, Daniel B.
通讯作者：
Kearns, Daniel B.
影响因子：
3.6
作者：
Chai Y;Kolter R;Losick R
通讯作者：
Losick R
影响因子：
3.2
作者：
ALBERTINI, AM;CARAMORI, T;GALIZZI, A
通讯作者：
GALIZZI, A