Paralogous antirepressors acting on the master regulator for biofilm formation in Bacillus subtilis.
Paralogous antirepressors acting on the master regulator for biofilm formation in Bacillus subtilis.
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DOI：
10.1111/j.1365-2958.2009.06900.x
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发表时间：
2009-11
影响因子：
3.6
通讯作者：
Losick R
中科院分区：
文献类型：
--
作者：
Chai Y;Kolter R;Losick R
枯草芽孢杆菌在生物膜形成过程中产生的基质是由一个基因控制电路控制的，该电路的核心是三个专门的调节蛋白，即抑制因子SINI、抑制因子SinR和下游调节因子SLrR。基质的产生是由SINI的合成触发的，SINI与SINR结合并使SINR失活，从而使基质产生的基因和SLrR的基因失活。最近，又发现了两个基质基因的调控因子：SLrA和YwcC，前者被认为是SLrR的激活因子，后者是SLrA合成的抑制因子。我们提出的证据表明SLrA是SINI的类似物，就像SINI一样，SINI是一种与SINR结合并使SINR失活的抗抑制药。我们还表明，slrA不能激活slrR来表达基质基因。相反，slrR结合并抑制slrA的活性。因此，YwcC-slrA-sinR-slrR途径是一个负反馈环，slrA间接刺激slrR的合成，slrR反过来抑制slrA的活性。最后，我们报告说，在标准的实验室条件下，slrA对基质产生的基因表达只有很小的贡献。我们认为，作为对YwcC抑制子识别的未知信号的反应，SLrA暂时促进了基质的产生。
Matrix production during biofilm formation by Bacillus subtilis is governed by a gene control circuit at the heart of which are three dedicated regulatory proteins, the antirepressor SinI, the repressor SinR, and the downstream regulator SlrR. Matrix production is triggered by the synthesis of SinI, which binds to and inactivates SinR, thereby derepressing genes for matrix production as well as the gene for SlrR. Recently, two additional regulators of matrix genes were identified: SlrA, which was reported to be an activator of SlrR, and YwcC, a repressor of SlrA synthesis. We present evidence indicating that SlrA, which is a paralog of SinI, is like SinI, an antirepressor that binds to, and inactivates, SinR. We also show that SlrA does not activate SlrR for expression of matrix genes. Instead, SlrR binds to, and inhibits the activity of, SlrA. Thus, the YwcC-SlrA-SinR-SlrR pathway is a negative feedback loop in which SlrA indirectly stimulates the synthesis of SlrR, and SlrR, in turn, inhibits the activity of SlrA. Finally, we report that under standard laboratory conditions SlrA makes only a small contribution to the expression of genes for matrix production. We propose that in response to an unknown signal recognized by the YwcC repressor, SlrA transiently boosts matrix production.
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影响因子：
64.5
作者：
BURBULYS, D;TRACH, KA;HOCH, JA
通讯作者：
HOCH, JA
影响因子：
3.6
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3.6
作者：
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通讯作者：
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