Components of coated vesicles and nuclear pore complexes share a common molecular architecture.
Components of coated vesicles and nuclear pore complexes share a common molecular architecture.
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DOI：
10.1371/journal.pbio.0020380
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发表时间：
2004-12
期刊：
影响因子：
9.8
通讯作者：
Rout MP
中科院分区：
文献类型：
--
作者：
Devos D;Dokudovskaya S;Alber F;Williams R;Chait BT;Sali A;Rout MP
原核生物与真核生物有许多不同的特征，其中最主要的是真核细胞独特的内膜系统。这些内膜系统形成了精细的区室和囊泡运输途径，并将染色质封闭在核膜内。核孔复合体是专门介导大分子穿过核膜运输的门户。虽然人们普遍认为这些内膜系统是由原核细胞质膜的特化内陷进化而来的，但核孔复合体如何从没有类似运输系统的生物体进化而来尚不清楚。在这里，我们利用计算和生化方法对构成yNup84/vNup107 - 160亚复合体的七种蛋白质进行结构分析，该亚复合体是核孔复合体的一个核心组成部分。我们的分析表明，所有七种蛋白质要么包含β - 螺旋桨折叠结构，要么包含α - 螺线管折叠结构，或者是两者的独特组合，这揭示了构成yNup84/vNup107 - 160亚复合体的结构与构成维持囊泡运输途径的主要类型的囊泡被膜复合体的结构之间的紧密相似性。这些相似性表明核孔复合体和被膜囊泡有共同的进化起源，即源于一个早期的膜弯曲模块，这个模块导致了现代真核生物内膜系统的形成。
核孔亚复合体的蛋白质与构成囊泡被膜复合体的蛋白质之间的结构相似性表明核孔复合体和被膜囊泡有共同的起源
Numerous features distinguish prokaryotes from eukaryotes, chief among which are the distinctive internal membrane systems of eukaryotic cells. These membrane systems form elaborate compartments and vesicular trafficking pathways, and sequester the chromatin within the nuclear envelope. The nuclear pore complex is the portal that specifically mediates macromolecular trafficking across the nuclear envelope. Although it is generally understood that these internal membrane systems evolved from specialized invaginations of the prokaryotic plasma membrane, it is not clear how the nuclear pore complex could have evolved from organisms with no analogous transport system. Here we use computational and biochemical methods to perform a structural analysis of the seven proteins comprising the yNup84/vNup107–160 subcomplex, a core building block of the nuclear pore complex. Our analysis indicates that all seven proteins contain either a β-propeller fold, an α-solenoid fold, or a distinctive arrangement of both, revealing close similarities between the structures comprising the yNup84/vNup107–160 subcomplex and those comprising the major types of vesicle coating complexes that maintain vesicular trafficking pathways. These similarities suggest a common evolutionary origin for nuclear pore complexes and coated vesicles in an early membrane-curving module that led to the formation of the internal membrane systems in modern eukaryotes. Structural similarities between the proteins of a nuclear pore subcomplex and proteins comprising vesicle coating complexes indicate a common origin for nuclear pore complexes and coated vesicles
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