A comparison of the Giardia lamblia trophozoite and cyst transcriptome using microarrays.
A comparison of the Giardia lamblia trophozoite and cyst transcriptome using microarrays.
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DOI：
10.1186/1471-2180-11-91
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发表时间：
2011-05-04
期刊：
影响因子：
4.2
通讯作者：
Widmer G
中科院分区：
文献类型：
--
作者：
Faghiri Z;Widmer G
与许多原生动物相比，蓝氏贾第鞭毛虫的生活史简单，在包囊和滋养体之间交替。对贾第鞭毛虫分子生物学的研究主要集中在滋养体及其胞外和囊化过程上，而对包囊的研究较少。休眠的包囊和快速分裂和活动的滋养体之间的显著形态差异表明，当寄生虫进入寄主的肠道并被新寄主摄取时，新陈代谢发生了深刻的变化。为了研究在蓝氏菌生活史中发生的转录变化的幅度，我们使用单色寡核苷酸微阵列比较了蓝氏菌滋养体和包囊的转录组。研究发现，无论是在mRNA的多样性还是丰度方面，包囊的转录组都要小得多。与核糖体功能相关的编码蛋白质的基因被高度过度表达。对培养产生的包囊和从粪便中提取的包囊的转录组进行比较，发现重叠很少，这增加了这两种包囊之间存在显著生物学差异的可能性。兰氏原虫包囊和滋养体转录组的比较表明，大多数基因的转录本在包囊中的表达水平较低。包囊和滋养体转录组的这一全球视角是对滋养体在繁殖、包囊和外结晶过程中所选基因表达的研究的补充。
Compared with many protists, Giardia lamblia has a simple life cycle alternating between cyst and trophozoite. Most research on the molecular biology of Giardia parasites has focused on trophozoites and the processes of excystation and encystation, whereas cysts have attracted less interest. The striking morphological differences between the dormant cyst and the rapidly dividing and motile trophozoite implies profound changes in the metabolism as the parasite encysts in the host's intestine and excysts upon ingestion by a new host. To investigate the magnitude of the transcriptional changes occurring during the G. lamblia life cycle we compared the transcriptome of G. lamblia trophozoites and cysts using single-color oligonucleotide microarrays. Cysts were found to possess a much smaller transcriptome, both in terms of mRNA diversity and abundance. Genes encoding proteins related to ribosomal functions are highly over-represented. The comparison of the transcriptome of cysts generated in culture or extracted from feces revealed little overlap, raising the possibility of significant biological differences between the two types of cysts. The comparison of the G. lamblia cyst and trophozoite transcriptome showed that transcripts of most genes are present at a lower level in cysts. This global view of the cyst and trophozoite transcriptome complements studies focused on the expression of selected genes during trophozoite multiplication, encystation and excystation.
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影响因子：
2
作者：
Gallego, Eva;Alvarado, Magda;Wasserman, Moises
通讯作者：
Wasserman, Moises
影响因子：
5.2
作者：
Mueller, Joachim;Ley, Serej;Mueller, Norbert
通讯作者：
Mueller, Norbert
影响因子：
4.4
作者：
SAUCH, JF;FLANIGAN, D;JAKUBOWSKI, W
通讯作者：
JAKUBOWSKI, W
影响因子：
3.6
作者：
Sonda, Sabrina;Morf, Laura;Hehl, Adrian B.
通讯作者：
Hehl, Adrian B.
影响因子：
56.9
作者：
GILLIN, FD;REINER, DS;SAUCH, JF
通讯作者：
SAUCH, JF