Ganjam virus/Nairobi sheep disease virus induces a pro-inflammatory response in infected sheep.
Ganjam virus/Nairobi sheep disease virus induces a pro-inflammatory response in infected sheep.
复制标题
DOI：
10.1186/1297-9716-43-71
复制
发表时间：
2012-10-19
影响因子：
4.4
通讯作者：
Baron MD
中科院分区：
文献类型：
--
作者：
Bin Tarif A;Lasecka L;Holzer B;Baron MD
部分由于气候变化，部分由于人类栖息地的改变，蜱传病毒的影响正在增加。内罗毕绵羊病病毒（NSDV）和甘贾姆病毒（GV）是同一种病毒的两个名称，这种病毒导致绵羊和山羊患病，目前已知在印度和东非流行。该病毒由硬蜱传播，并导致严重的出血性疾病。我们已经开发了一种实时PCR检测病毒基因组，并验证了它在两个不同的分离株的NSDV/GV诱导的致病性的初步研究。一个分离株是高度适应组织培养，生长在大多数细胞系测试，基本上是在羊的致病性。第二个分离株似乎是不适应细胞培养，并保留在羊的致病性。病毒的实时PCR检测很容易检测到4个拷贝或更少的病毒基因组，并允许定量测量全血中的病毒。对细胞因子mRNA变化的测量显示，与在被密切相关的病毒克里米亚刚果出血热病毒感染的人类中观察到的变化相似。
Partly due to climate change, and partly due to changes of human habitat occupation, the impact of tick-borne viruses is increasing. Nairobi sheep disease virus (NSDV) and Ganjam virus (GV) are two names for the same virus, which causes disease in sheep and goats and is currently known to be circulating in India and East Africa. The virus is transmitted by ixodid ticks and causes a severe hemorrhagic disease. We have developed a real-time PCR assay for the virus genome and validated it in a pilot study of the pathogenicity induced by two different isolates of NSDV/GV. One isolate was highly adapted to tissue culture, grew in most cell lines tested, and was essentially apathogenic in sheep. The second isolate appeared to be poorly adapted to cell culture and retained pathogenicity in sheep. The real-time PCR assay for virus easily detected 4 copies or less of the viral genome, and allowed a quantitative measure of the virus in whole blood. Measurement of the changes in cytokine mRNAs showed similar changes to those observed in humans infected by the closely related virus Crimean Congo hemorrhagic fever virus.
登录
查看更多内容
影响因子：
3.8
作者：
Li, Hong;Cunha, Cristina W.;Taus, Naomi S.
通讯作者：
Taus, Naomi S.
影响因子：
10.4
作者：
RYAN, US
通讯作者：
RYAN, US
影响因子：
4.4
作者：
Hensley, LE;Young, HA;Geisbert, TW
通讯作者：
Geisbert, TW
影响因子：
11.8
作者：
Karti SS;Odabasi Z;Korten V;Yilmaz M;Sonmez M;Caylan R;Akdogan E;Eren N;Koksal I;Ovali E;Erickson BR;Vincent MJ;Nichol ST;Comer JA;Rollin PE;Ksiazek TG
通讯作者：
Ksiazek TG
影响因子：
2.6
作者：
Smeed, Jennifer A;Watkins, Craig A;Hopkins, John
通讯作者：
Hopkins, John