The Rx transcription factor is required for determination of the retinal lineage and regulates the timing of neuronal differentiation
The Rx transcription factor is required for determination of the retinal lineage and regulates the timing of neuronal differentiation
复制标题
Rx 转录因子是确定视网膜谱系和调节神经元分化时间所必需的
DOI：
10.1111/dgd.12796
复制
发表时间：
2022
影响因子：
2.5
通讯作者：
Noriaki Sasai
中科院分区：
文献类型：
--
作者：
Maho Yamamoto;Agnes Ong Lee Chen;Takuma Shinozuka;Noriaki Sasai
了解导致视网膜发育的分子机制对于基础科学和临床应用都具有极大的兴趣。已经分离和分析了参与视网膜发育的几种信号分子和转录因子;然而，由于难以识别不同个体中相应的细胞谱系，确定特定分子丢失的直接影响是有问题的。在这里，我们进行了全基因组表达分析与胚胎干细胞（ES）细胞缺乏的Rx基因，它编码的几个同源框转录因子之一，视网膜发育必不可少的。我们对野生型和突变型细胞进行了三维分化，并比较了它们的基因表达谱。突变组织未能分化成视网膜谱系，并表现出神经元细胞特征基因的早熟表达。总之，这些结果表明Rx表达是视网膜谱系的重要生物标志物，并且它有助于调节适当的分化阶段。
Understanding the molecular mechanisms leading to retinal development is of great interest for both basic scientific and clinical applications. Several signaling molecules and transcription factors involved in retinal development have been isolated and analyzed; however, determining the direct impact of the loss of a specific molecule is problematic, due to difficulties in identifying the corresponding cellular lineages in different individuals. Here, we conducted genome‐wide expression analysis with embryonic stem (ES) cells devoid of theRxgene, which encodes one of several homeobox transcription factors essential for retinal development. We performed three‐dimensional differentiation of wild‐type and mutant cells and compared their gene‐expression profiles. The mutant tissue failed to differentiate into the retinal lineage and exhibited precocious expression of genes characteristic of neuronal cells. Together, these results suggest that Rx expression is an important biomarker of the retinal lineage and that it helps regulates appropriate differentiation stages.
登录
查看更多内容
影响因子：
3.5
作者：
Lequeux, L.;Rio, M.;Calvas, P.
通讯作者：
Calvas, P.
影响因子：
10.5
作者：
Zhang, Chun-Li;Zou, Yuhua;Evans, Ronald M.
通讯作者：
Evans, Ronald M.
影响因子：
11.8
作者：
Pera, EM;Wessely, O;De Robertis, EM
通讯作者：
De Robertis, EM
锌指基因 Fezf1 和 Fezf2 通过抑制前脑 Hes5 表达来控制神经元分化
影响因子：
4.6
作者：
Shimizu;T.
通讯作者：
T.
影响因子：
64.8
作者：
Mathers, PH;Grinberg, A;Jamrich, M
通讯作者：
Jamrich, M