Surveying silicon nitride nanopores for glycomics and heparin quality assurance.
Surveying silicon nitride nanopores for glycomics and heparin quality assurance.
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DOI：
10.1038/s41467-018-05751-y
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发表时间：
2018-08-16
影响因子：
16.6
通讯作者：
Dwyer JR
中科院分区：
文献类型：
--
作者：
Karawdeniya BI;Bandara YMNDY;Nichols JW;Chevalier RB;Dwyer JR
多糖具有关键的生物学功能，可以用于治疗作用，如抗凝肝素。它们的复杂性-例如，超过100种单糖，具有多种连接和分支结构-与其他生物聚合物（如DNA和蛋白质）相比，大大增加了分析的复杂性。更多的，改进的，分析工具已经被要求，在这里，我们证明，固态氮化硅纳米孔传感器和调谐传感条件可用于可靠地检测天然多糖和酶消化产物，在简单的测定中区分不同的多糖，提供新的实验见解纳米孔电动力学，并揭示多糖的性质。我们表明，纳米孔传感使我们能够很容易地区分临床肝素样品和2008年导致死亡的污染物，当其存在未被传统检测方法检测到时。本文报道的工作为进一步探索多糖表征和开发使用薄膜固态纳米孔传感器的测定奠定了基础。与其他生物聚合物相比，多糖的复杂性使其分析显着复杂化。在这里，作者证明了固态氮化硅纳米孔传感器可用于可靠地检测天然多糖，并对多糖治疗剂肝素进行简单的质量保证测定。
Polysaccharides have key biological functions and can be harnessed for therapeutic roles, such as the anticoagulant heparin. Their complexity—e.g., >100 monosaccharides with variety in linkage and branching structure—significantly complicates analysis compared to other biopolymers such as DNA and proteins. More, and improved, analysis tools have been called for, and here we demonstrate that solid-state silicon nitride nanopore sensors and tuned sensing conditions can be used to reliably detect native polysaccharides and enzymatic digestion products, differentiate between different polysaccharides in straightforward assays, provide new experimental insights into nanopore electrokinetics, and uncover polysaccharide properties. We show that nanopore sensing allows us to easily differentiate between a clinical heparin sample and one spiked with the contaminant that caused deaths in 2008 when its presence went undetected by conventional assays. The work reported here lays a foundation to further explore polysaccharide characterization and develop assays using thin-film solid-state nanopore sensors. The complexity of polysaccharides significantly complicates their analysis in comparison to other biopolymers. Here, the authors demonstrate that solid-state silicon nitride nanopore sensors can be used to reliably detect native polysaccharides and to perform a simple quality assurance assay on a polysaccharide therapeutic, heparin.
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影响因子：
7.4
作者：
Haywood, Daniel G.;Saha-Shah, Anumita;Baker, Lane A.;Jacobson, Stephen C.
通讯作者：
Jacobson, Stephen C.
通过识别隧道效应对碳水化合物异构体进行电子单分子识别。
影响因子：
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作者：
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影响因子：
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通讯作者：
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作者：
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通讯作者：
Sasisekharan, Ram
影响因子：
4.3
作者：
DeMarco ML;Woods RJ
通讯作者：
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