Low expression of miR-142-3p promotes intervertebral disk degeneration.
Low expression of miR-142-3p promotes intervertebral disk degeneration.
复制标题
MiR-142-3p低表达促进椎间盘退变。
DOI：
10.1186/s13018-020-02194-4
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发表时间：
2021-01-14
影响因子：
2.6
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Xue J;Hu B;Xing W;Li F;Huang Z;Zheng W;Wang B;Zhu Y;Yang X
椎间盘退变是一种以细胞质丢失和细胞外基质降解为特征的退行性疾病。大量证据表明miRNAs参与了IDD的发生发展。然而，miR-142- 3 p在IDD发展中的功能仍然未知。本研究主要探讨miR-142- 3 p在IDD发生发展中的潜在作用。用1%胎牛血清诱导ATDC 5细胞变性，qRT-PCR检测miR-142- 3 p水平。然后，将miR-142- 3 p模拟物/抑制剂及其相应的阴性对照转染到ATDC 5正常和变性细胞中。分别使用CCK 8、伤口愈合试验、annexin V-FITC/PI染色、western blot和qRT-PCR评估存活力、迁移、侵袭、凋亡、周期、Bax、Bcl-2、P62和Beclin 1表达水平。结果显示，ATDC 5变性细胞中MMP 13、ADAMTS 5、MMP 3和Col-X的表达水平升高，SOX-9和Col-II的表达水平降低，表明建立了变性模型。我们观察到miR-142- 3 p在ATDC 5退变细胞中减少，其抑制可促进ATDC 5细胞退变。然而，miR-142- 3 p过表达可逆转ATDC 5变性细胞的细胞活力抑制，以及凋亡和自噬增强。我们的研究结果证明miR-142- 3 p可能在椎间盘退变中起重要作用。需要进一步的动物研究来说明miR-142- 3 p在IDD发展中的作用。在线版本包含补充材料，可通过10.1186/s13018-020-02194-4获得。
Intervertebral disk degeneration (IDD) is a degenerative disease characterized by cytoplasm loss and extracellular matrix degradation. Numerous evidence reported that miRNAs participated in IDD development. Nevertheless, the function of miR-142-3p in IDD development remains unknown. This study mainly explored the potential role and function of miR-142-3p in IDD development. One percent fetal bovine serum was used to induce the degeneration of ATDC5 cells, and miR-142-3p level was examined by qRT-PCR. Then, miR-142-3p mimic/inhibitor and its corresponding negative control were transfected into ATDC5 normal and degenerative cells. Viability, migration, invasion, apoptosis, cycle, Bax, Bcl-2, P62, and Beclin1 expression levels were assessed using CCK8, wound healing assay, annexin V-FITC/PI staining, western blot, and qRT-PCR, respectively. The results revealed that the expression levels of MMP13, ADAMTS5, MMP3, and Col-X were increased as well as the expression levels of SOX-9 and Col-II were reduced in ATDC5 degenerative cells, indicating the degeneration model was constructed. We observed that miR-142-3p was decreased in ATDC5 degenerative cells and its suppression could promote ATDC5 cell degeneration. However, miR-142-3p overexpression could reverse the cell viability inhibition, as well as apoptosis and autophagy enhancement in ATDC5 degenerative cells. Our results proved that miR-142-3p may play an important role in disk degeneration. Further animal study is needed to illustrate the role of the miR-142-3p in IDD development. The online version contains supplementary material available at 10.1186/s13018-020-02194-4.
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影响因子：
7.4
作者：
Wu A;Dong W;Liu S;Cheung JPY;Kwan KYH;Zeng X;Zhang K;Sun Z;Wang X;Cheung KMC;Zhou M;Zhao J
通讯作者：
Zhao J
影响因子：
5.7
作者：
Winer A;Adams S;Mignatti P
通讯作者：
Mignatti P
影响因子：
4
作者：
Qiang, Jianhong;Lv, Tingting;Yang, Xichao
通讯作者：
Yang, Xichao
影响因子：
4
作者：
Wang, Jingjie;Liu, Xiaoyan;Sun, Yuanliang
通讯作者：
Sun, Yuanliang
影响因子：
4.2
作者：
Yamasaki, A;Itabashi, M;Nagai, N
通讯作者：
Nagai, N