SCF increases cardiac stem cell migration through PI3K/AKT and MMP‑2/‑9 signaling.
SCF increases cardiac stem cell migration through PI3K/AKT and MMP‑2/‑9 signaling.
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DOI：
10.3892/ijmm.2014.1773
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发表时间：
2014-07
影响因子：
5.4
通讯作者：
Zheng S
中科院分区：
文献类型：
--
作者：
Guo J;Jie W;Shen Z;Li M;Lan Y;Kong Y;Guo S;Li T;Zheng S
The transplantation of cardiac stem cells (CSCs) is thought to be responsible for improving the performance of injured heart induced by myocardial infarction (MI). However, the mechanisms involved in the migration of activated CSCs post-MI remain to be clarified. In this study, CSCs were isolated from rat hearts and a cellular migration assay was performed using a 24-well Transwell system. Stem cell factor (SCF) induced CSC migration in a concentration-dependent manner, which could be blocked with an SCF antibody as well as a PI3K/AKT inhibitor, LY294002. Moreover, SCF induced the expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9 in a concentration- and time-dependent manner, as measured by quantitative RT-PCR, western blot analysis and gelatin zymography. Results of western blot analysis revealed phosphorylated AKT was markedly increased in SCF-treated CSCs and that inhibition of SCF/c-Kit signaling or phospho-AKT activity significantly attenuated the SCF-induced expression of MMP-2 and MMP-9. Thus, our results showed that SCF partially mediated CSC migration via the activation of PI3K/AKT/MMP-2/-9 signaling.
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影响因子：
168.9
作者：
Makkar, Raj R.;Smith, Rachel R.;Cheng, Ke;Malliaras, Konstantinos;Thomson, Louise E. J.;Berman, Daniel;Czer, Lawrence S. C.;Marban, Linda;Mendizabal, Adam;Johnston, Peter V.;Russell, Stuart D.;Schuleri, Karl H.;Lardo, Albert C.;Gerstenblith, Gary;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
10.8
作者：
Lutz, Matthias;Rosenberg, Mark;Frey, Norbert
通讯作者：
Frey, Norbert
影响因子：
37.8
作者：
Bolli R;Tang XL;Sanganalmath SK;Rimoldi O;Mosna F;Abdel-Latif A;Jneid H;Rota M;Leri A;Kajstura J
通讯作者：
Kajstura J
影响因子：
9.2
作者：
Liang J;Wu YL;Chen BJ;Zhang W;Tanaka Y;Sugiyama H
通讯作者：
Sugiyama H
影响因子：
64.5
作者：
Ellison, Georgina M.;Vicinanza, Carla;Nadal-Ginard, Bernardo
通讯作者：
Nadal-Ginard, Bernardo