Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning.
Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning.
复制标题
DOI：
10.1371/journal.pone.0041536
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Arregui CO
中科院分区：
文献类型：
--
作者：
Fuentes F;Zimmer D;Atienza M;Schottenfeld J;Penkala I;Bale T;Bence KK;Arregui CO
ER-bound PTP1B is expressed in hippocampal neurons, and accumulates among neurite contacts. PTP1B dephosphorylates ß-catenin in N-cadherin complexes ensuring cell-cell adhesion. Here we show that endogenous PTP1B, as well as expressed GFP-PTP1B, are present in dendritic spines of hippocampal neurons in culture. GFP-PTP1B overexpression does not affect filopodial density or length. In contrast, impairment of PTP1B function or genetic PTP1B-deficiency leads to increased filopodia-like dendritic spines and a reduction in mushroom-like spines, while spine density is unaffected. These morphological alterations are accompanied by a disorganization of pre- and post-synapses, as judged by decreased clustering of synapsin-1 and PSD-95, and suggest a dynamic synaptic phenotype. Notably, levels of ß-catenin-Tyr-654 phosphorylation increased ∼5-fold in the hippocampus of adult PTP1B−/− (KO) mice compared to wild type (WT) mice and this was accompanied by a reduction in the amount of ß-catenin associated with N-cadherin. To determine whether PTP1B-deficiency alters learning and memory, we generated mice lacking PTP1B in the hippocampus and cortex (PTP1Bfl/fl–Emx1-Cre). PTP1Bfl/fl–Emx1-Cre mice displayed improved performance in the Barnes maze (decreased time to find and enter target hole), utilized a more efficient strategy (cued), and had better recall compared to WT controls. Our results implicate PTP1B in structural plasticity within the hippocampus, likely through modulation of N-cadherin function by ensuring dephosphorylation of ß-catenin on Tyr-654. Disruption of hippocampal PTP1B function or expression leads to elongation of dendritic filopodia and improved learning and memory, demonstrating an exciting novel role for this phosphatase.
登录
查看更多内容
影响因子：
15.9
作者：
Banno, Ryoichi;Zimmer, Derek;Bence, Kendra K.
通讯作者：
Bence, Kendra K.
突触时的钙粘蛋白和Catenin：突触发生和突触可塑性中的作用。
影响因子：
15.9
作者：
Arikkath, Jyothi;Reichardt, Louis F.
通讯作者：
Reichardt, Louis F.
DOI：
10.1037/h0077579
发表时间：
1979-01-01
期刊：
JOURNAL OF COMPARATIVE AND PHYSIOLOGICAL PSYCHOLOGY
影响因子：
--
作者：
BARNES, CA
通讯作者：
BARNES, CA
影响因子：
11.2
作者：
Bentires-Alj, Mohamed;Neel, Benjamin G.
通讯作者：
Neel, Benjamin G.
影响因子：
16.2
作者：
Bamji, SX;Shimazu, K;Reichardt, LF
通讯作者：
Reichardt, LF