Glutathione supplementation suppresses muscle fatigue induced by prolonged exercise via improved aerobic metabolism.
Glutathione supplementation suppresses muscle fatigue induced by prolonged exercise via improved aerobic metabolism.
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DOI：
10.1186/s12970-015-0067-x
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发表时间：
2015
影响因子：
5.1
通讯作者：
Higashi A
中科院分区：
文献类型：
--
作者：
Aoi W;Ogaya Y;Takami M;Konishi T;Sauchi Y;Park EY;Wada S;Sato K;Higashi A
谷胱甘肽是动物细胞内的一种内源性氧化还原偶联，在抗氧化防御和解毒中发挥重要作用，但目前尚不清楚口服谷胱甘肽是否会影响运动诱导的生理变化。本研究探讨了摄入谷胱甘肽对小鼠和人类运动诱导的肌肉代谢和疲劳的影响。将ICR小鼠分为4组：久坐对照组、久坐添加谷胱甘肽（2.0%，5 μL/g体重）组、运动对照组和运动添加谷胱甘肽组。2周后，运动组在跑步机上以25米/分钟的速度跑步30分钟。运动后立即测量肌间pH值，并采集后肢肌肉和血液样本测量生化参数。在一项双盲交叉研究中，8名健康男性（35.9±2.0岁）被给予谷胱甘肽（1 g/d）或安慰剂2周。然后，他们以40%的最大心率在自行车计力器上锻炼60分钟。运动后检测心理状态及血液生化指标。小鼠实验中，运动添加谷胱甘肽组运动后血浆非酯化脂肪酸含量（820±44 mEq/L）显著低于运动对照组（1152±61 mEq/L）。肌间pH值随运动降低（7.17±0.01）；然而，这种减少被补充谷胱甘肽（7.23±0.02）所阻止。与久坐对照组相比，久坐补充谷胱甘肽组过氧化物酶体增殖体激活受体-γ辅激活因子-1α蛋白和线粒体DNA水平显著升高（分别高25%和53%）。在人体研究中，摄入谷胱甘肽可抑制血乳酸升高（安慰剂，3.4±1.1 mM；谷胱甘肽，2.9±0.6 mM）。与安慰剂试验相比，谷胱甘肽试验显著降低了与疲劳相关的心理因素。这些结果表明，补充谷胱甘肽可以改善运动期间骨骼肌的脂质代谢和酸化，从而减少肌肉疲劳。本文的在线版本（doi:10.1186/s12970-015-0067-x）包含补充材料，可供授权用户使用。
Glutathione is an endogenous redox couple in animal cells and plays important roles in antioxidant defense and detoxification, although it is unknown if oral glutathione supplementation affects exercise-induced physiological changes. The present study investigated the effect of glutathione intake on exercise-induced muscle metabolism and fatigue in mice and humans. ICR mice were divided into 4 groups: sedentary control, sedentary supplemented with glutathione (2.0%, 5 μL/g body weight), exercise control, and exercise supplemented with glutathione. After 2 weeks, the exercise groups ran on a treadmill at 25 m/min for 30 min. Immediately post-exercise, intermuscular pH was measured, and hind limb muscle and blood samples were collected to measure biochemical parameters. In a double-blind, cross-over study, 8 healthy men (35.9 ± 2.0 y) were administered either glutathione (1 g/d) or placebo for 2 weeks. Then, they exercised on a cycle ergometer at 40% maximal heart rate for 60 min. Psychological state and blood biochemical parameters were examined after exercise. In the mouse experiment, post-exercise plasma non-esterified fatty acids were significantly lower in the exercise supplemented with glutathione group (820 ± 44 mEq/L) compared with the exercise control group (1152 ± 61 mEq/L). Intermuscular pH decreased with exercise (7.17 ± 0.01); however, this reduction was prevented by glutathione supplementation (7.23 ± 0.02). The peroxisome proliferator-activated receptor-γ coactivator-1α protein and mitochondrial DNA levels were significantly higher in the sedentary supplemented with glutathione group compared with the sedentary control group (25% and 53% higher, respectively). In the human study, the elevation of blood lactate was suppressed by glutathione intake (placebo, 3.4 ± 1.1 mM; glutathione, 2.9 ± 0.6 mM). Fatigue-related psychological factors were significantly decreased in the glutathione trial compared with the placebo trial. These results suggest that glutathione supplementation improved lipid metabolism and acidification in skeletal muscles during exercise, leading to less muscle fatigue. The online version of this article (doi:10.1186/s12970-015-0067-x) contains supplementary material, which is available to authorized users.
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影响因子：
29
作者：
Zechner C;Lai L;Zechner JF;Geng T;Yan Z;Rumsey JW;Collia D;Chen Z;Wozniak DF;Leone TC;Kelly DP
通讯作者：
Kelly DP
DOI：
10.1073/pnas.1032913100
发表时间：
2003-07-08
影响因子：
11.1
作者：
Patti, ME;Butte, AJ;Mandarino, LJ
通讯作者：
Mandarino, LJ
影响因子：
3.4
作者：
Gambelunghe, C;Rossi, R;Rufini, S
通讯作者：
Rufini, S
DOI：
10.1073/pnas.0911570106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Wenz, Tina;Rossi, Susana G.;Moraes, Carlos T.
通讯作者：
Moraes, Carlos T.
DOI：
10.1073/pnas.0903485106
发表时间：
2009-05-26
影响因子：
11.1
作者：
Ristow, Michael;Zarse, Kim;Blueher, Matthias
通讯作者：
Blueher, Matthias