RNA is required for the integrity of multiple nuclear and cytoplasmic membrane-less RNP granules.
RNA is required for the integrity of multiple nuclear and cytoplasmic membrane-less RNP granules.
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DOI：
10.15252/embj.2021110137
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发表时间：
2022-05-02
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影响因子：
--
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在真核细胞的细胞核和细胞质中观察到许多由RNA和蛋白质组合组成的无膜细胞器。这些RNP颗粒包括应激颗粒（SG）、加工体（PB）、Cajal小体和核斑点。一个尚未解决的问题是，在细胞核或胞质溶胶中，RNP颗粒的完整性需要RNA分子的频率。为了解决这个问题，我们降解细胞内RNA在细胞质或细胞核中的激活RNase L和检查的影响RNA损失的几个RNP颗粒。我们发现大多数的RNP颗粒，包括SGs，Cajal小体，核斑点，和核仁，被改变的降解其RNA成分。相比之下，PB和超级增强子复合物在很大程度上不受各自隔室中RNA降解的影响。RNA降解总体上导致一些无膜细胞器的明显溶解，而其他细胞器重组成具有改变的形态的结构。这些发现突出了RNA在几个RNP颗粒的组织中的关键和广泛的作用。细胞核或细胞质RNA的靶向降解揭示了RNA在维持特定RNP颗粒中的不同作用。
Numerous membrane‐less organelles, composed of a combination of RNA and proteins, are observed in the nucleus and cytoplasm of eukaryotic cells. These RNP granules include stress granules (SGs), processing bodies (PBs), Cajal bodies, and nuclear speckles. An unresolved question is how frequently RNA molecules are required for the integrity of RNP granules in either the nucleus or cytosol. To address this issue, we degraded intracellular RNA in either the cytosol or the nucleus by the activation of RNase L and examined the impact of RNA loss on several RNP granules. We find the majority of RNP granules, including SGs, Cajal bodies, nuclear speckles, and the nucleolus, are altered by the degradation of their RNA components. In contrast, PBs and super‐enhancer complexes were largely not affected by RNA degradation in their respective compartments. RNA degradation overall led to the apparent dissolution of some membrane‐less organelles, whereas others reorganized into structures with altered morphology. These findings highlight a critical and widespread role of RNA in the organization of several RNP granules. Targeted degradation of nuclear or cytoplasmic RNA uncovers differential roles for RNA in maintaining specific RNP granules.
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DOI：
10.1083/jcb.202009079
发表时间：
2021-03-01
期刊：
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影响因子：
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通讯作者：
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