The CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes.
The CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes.
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CMG（CDC45/RECJ，MCM，GINS）复合物是所有古细菌和真核生物中DNA复制系统的保守组成部分。
DOI：
10.1186/1745-6150-7-7
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发表时间：
2012-02-13
期刊：
影响因子：
5.5
通讯作者：
Kelman Z
中科院分区：
文献类型：
--
作者：
Makarova KS;Koonin EV;Kelman Z
在真核生物中，含有复制解旋酶MCM的CMG（CDC 45，MCM，GINS）复合物是DNA复制的关键参与者。真核细胞MCM和GINS蛋白的同源物已经被鉴定，但直到最近还不知道CDC 45蛋白的同源物。两个最近的发展，即古菌GINS相关核酸酶（GAN），属于DHH水解酶超家族的RecJ家族的发现和CDC 45和RecJ的DHH结构域之间的同源性的证明，表明至少有一些古菌具有CMG复合物亚基的同源物的完整互补。在这里，我们提出了深入的RecJ同源基因组分析的结果在古细菌。我们证实并扩展了最近的假设，CDC 45是真核生物的细菌和古细菌RecJ家族核酸酶的直系同源物。在所有测序的古细菌基因组中，除了Caldivirga maquilingensis之外，至少有一个RecJ同源物被确定。这些蛋白质包括以前未被注意的远程RecJ同源物与失活的DHH结构域在热蛋白目。结合不同的真核生物，古细菌和细菌DHH亚家族的系统发育树重建，这种分析产生了一个复杂的情况下RecJ家族的进化，其中包括独立的核酸酶结构域的Crenarchaeota和嗜盐菌的失活，并在甲烷球菌这个域的损失。古菌复合物的CDC 45/RecJ同源物，MCM和GINS是同源的，最有可能的功能类似于真核CMG复合物，似乎是一个关键组成部分的DNA复制机制，在所有的大肠杆菌。据推断，最后一个共同的古真核祖先编码的CMG复合物，其中包含一个活性核酸酶的RecJ家族。几个古细菌谱系中的失活RecJ同源物最有可能是复制复合物的专用结构组分。这篇文章由帕特里克·福特尔教授、斯蒂芬·约翰·阿维斯博士（由Purificacion Lopez-Garcia博士提名）和马丁·休恩教授审查。有关完整的评论，请参阅评论者的评论部分。
In eukaryotes, the CMG (CDC45, MCM, GINS) complex containing the replicative helicase MCM is a key player in DNA replication. Archaeal homologs of the eukaryotic MCM and GINS proteins have been identified but until recently no homolog of the CDC45 protein was known. Two recent developments, namely the discovery of archaeal GINS-associated nuclease (GAN) that belongs to the RecJ family of the DHH hydrolase superfamily and the demonstration of homology between the DHH domains of CDC45 and RecJ, show that at least some Archaea possess a full complement of homologs of the CMG complex subunits. Here we present the results of in-depth phylogenomic analysis of RecJ homologs in archaea. We confirm and extend the recent hypothesis that CDC45 is the eukaryotic ortholog of the bacterial and archaeal RecJ family nucleases. At least one RecJ homolog was identified in all sequenced archaeal genomes, with the single exception of Caldivirga maquilingensis. These proteins include previously unnoticed remote RecJ homologs with inactivated DHH domain in Thermoproteales. Combined with phylogenetic tree reconstruction of diverse eukaryotic, archaeal and bacterial DHH subfamilies, this analysis yields a complex scenario of RecJ family evolution in Archaea which includes independent inactivation of the nuclease domain in Crenarchaeota and Halobacteria, and loss of this domain in Methanococcales. The archaeal complex of a CDC45/RecJ homolog, MCM and GINS is homologous and most likely functionally analogous to the eukaryotic CMG complex, and appears to be a key component of the DNA replication machinery in all Archaea. It is inferred that the last common archaeo-eukaryotic ancestor encoded a CMG complex that contained an active nuclease of the RecJ family. The inactivated RecJ homologs in several archaeal lineages most likely are dedicated structural components of replication complexes. This article was reviewed by Prof. Patrick Forterre, Dr. Stephen John Aves (nominated by Dr. Purificacion Lopez-Garcia) and Prof. Martijn Huynen. For the full reviews, see the Reviewers' Comments section.
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DOI：
10.1126/science.1174343
发表时间：
2009-08-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Guo M;Chong YE;Beebe K;Shapiro R;Yang XL;Schimmel P
通讯作者：
Schimmel P
影响因子：
5.5
作者：
Makarova KS;Sorokin AV;Novichkov PS;Wolf YI;Koonin EV
通讯作者：
Koonin EV
影响因子：
14.9
作者：
Fang M;Zeisberg WM;Condon C;Ogryzko V;Danchin A;Mechold U
通讯作者：
Mechold U
影响因子：
56.9
作者：
FITCH, WM;MARGOLIASH, E
通讯作者：
MARGOLIASH, E
影响因子：
5.8
作者：
Cuff, JA;Clamp, ME;Barton, GJ
通讯作者：
Barton, GJ