Wnt5a regulates growth, patterning, and odontoblast differentiation of developing mouse tooth.
Wnt5a regulates growth, patterning, and odontoblast differentiation of developing mouse tooth.
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Wnt5a 调节发育中小鼠牙齿的生长、图案化和成牙本质细胞分化
DOI：
10.1002/dvdy.22550
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发表时间：
2011-02
影响因子：
2.5
通讯作者：
Chen, YiPing
中科院分区：
文献类型：
--
作者：
Lin, Minkui;Li, Lu;Liu, Chao;Liu, Hongbing;He, Fenglei;Yan, Fuhua;Zhang, Yanding;Chen, YiPing
Wnt/β-catenin信号通路在牙胚发育过程中起重要作用，但非经典Wnt信号通路在牙胚发育中的作用尚不清楚。在这里，我们比较了Wnt 5a的表达，一个代表性的非经典Wnt，与Ror 2，Wnt 5a受体的非经典信号，在发育中的牙齿，并分析牙齿表型的Wnt 5a突变体。Wnt 5a缺陷小鼠从E16.5开始表现出牙齿发育迟缓，导致出生时形成较小和异常图案的牙齿，并延迟成牙本质细胞分化。这些缺陷与牙上皮中Axin 2和Shh表达上调以及牙上皮和间充质中细胞增殖水平降低相关。在Ror 2突变小鼠中也观察到牙齿发育迟缓和成牙本质细胞分化缺陷。我们的研究结果表明，Wnt 5a调节生长，图案，成牙本质细胞分化在牙发育过程中，至少部分通过调节Wnt/β-catenin经典信号。
Wnt/β-catenin signaling is essential for tooth development beyond the bud stage, but little is known about the role of non-canonical Wnt signaling in odontogenesis. Here we compared the expression of Wnt5a, a representative of noncanonical Wnts, with that of Ror2, the Wnt5a receptor for non-canonical signaling, in the developing tooth, and analyzed tooth phenotype in Wnt5a mutants. Wnt5a deficient mice exhibit retarded tooth development beginning from E16.5, leading to the formation of smaller and abnormally patterned teeth with a delayed odontoblast differentiation at birth. These defects are associated with upregulated Axin2 and Shh expression in the dental epithelium and reduced levels of cell proliferation in the dental epithelium and mesenchyme. Retarded tooth development and defective odontoblast differentiation were also observed in Ror2 mutant mice. Our results suggest that Wnt5a regulates growth, patterning, and odontoblast differentiation during odontogenesis, at least partially by modulating Wnt/β-catenin canonical signaling.
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影响因子：
7.6
作者：
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通讯作者：
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作者：
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