Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines.
Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines.
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DOI：
10.1007/s00249-016-1173-7
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发表时间：
2016-10
影响因子：
2
通讯作者：
Prevarskaya, Natalia
中科院分区：
文献类型：
--
作者：
Iamshanova, Oksana;Mariot, Pascal;Lehen'kyi, V'yacheslav;Prevarskaya, Natalia
众所周知，钠离子（Na+）调节了许多涉及生理和病理条件的信号通路。特别是，细胞内Na+浓度的改变和相应的膜电位的变化被认为是癌症进展到转移表型的主要因素。虽然膜片钳技术可以研究Na+通道的功能和相应的Na+电流，但与Na+荧光成像相比，膜片钳技术对细胞内Na+瞬态的研究具有较大的侵入性和技术难度。尽管Na+信号被认为是癌症进展的重要控制因素，但迄今为止使用Na+成像方法的数据很少，这表明科学界存在持续的挑战。在这项研究中，我们详细描述了应用Na+成像技术测量人前列腺癌细胞细胞内Na+变化的方法。因此，我们使用了三种Na+特异性荧光染料：Na+结合苯并呋喃异眼酸盐（SBFI）、CoroNa™Green （CoroNa）和Asante NaTRIUM Green-2 （ANG-2）。对这些染料进行了最佳加载条件、不同校准方法后的解离常数和工作范围以及细胞内Na+灵敏度的评估，以确定哪种探针可以被认为是最可靠的体外Na+波动可视化。
Sodium (Na+) ions are known to regulate many signaling pathways involved in both physiological and pathological conditions. In particular, alterations in intracellular concentrations of Na+ and corresponding changes in membrane potential are known to be major actors of cancer progression to metastatic phenotype. Though the functionality of Na+ channels and the corresponding Na+ currents can be investigated using the patch-clamp technique, the latter is rather invasive and a technically difficult method to study intracellular Na+ transients compared to Na+ fluorescence imaging. Despite the fact that Na+ signaling is considered an important controller of cancer progression, only few data using Na+ imaging approaches are available so far, suggesting the persisting challenge within the scientific community. In this study, we describe in detail the approach for application of Na+ imaging technique to measure intracellular Na+ variations in human prostate cancer cells. Accordingly, we used three Na+-specific fluorescent dyes–Na+-binding benzofuran isophthalate (SBFI), CoroNa™ Green (Corona) and Asante NaTRIUM Green-2 (ANG-2). These dyes have been assessed for optimal loading conditions, dissociation constant and working range after different calibration methods, and intracellular Na+ sensitivity, in order to determine which probe can be considered as the most reliable to visualize Na+ fluctuations in vitro.
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影响因子：
3.8
作者：
Jacobs, Michael A.;Ouwerkerk, Ronald;Stearns, Vered
通讯作者：
Stearns, Vered
调节癌症电压门控通道表达：激素，生长因子和自身调节。
DOI：
10.1098/rstb.2013.0105
发表时间：
2014-03-19
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Fraser SP;Ozerlat-Gunduz I;Brackenbury WJ;Fitzgerald EM;Campbell TM;Coombes RC;Djamgoz MB
通讯作者：
Djamgoz MB
影响因子：
5.6
作者：
Fraser, SP;Salvador, V;Djamgoz, MBA
通讯作者：
Djamgoz, MBA
影响因子：
3
作者：
Meier, Silke D.;Kovalchuk, Yury;Rose, Christine R.
通讯作者：
Rose, Christine R.
影响因子：
8.3
作者：
Komlosi, P;Fuson, AL;Bell, PD
通讯作者：
Bell, PD