Localization of Nopp140 within mammalian cells during interphase and mitosis.
Localization of Nopp140 within mammalian cells during interphase and mitosis.
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DOI：
10.1007/s00418-009-0599-8
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发表时间：
2009-08
影响因子：
2.3
通讯作者：
Ploton, Dominique
中科院分区：
文献类型：
--
作者：
Thiry, Marc;Cheutin, Thierry;Lamaye, Francoise;Thelen, Nicolas;Meier, U. Thomas;O'Donohue, Marie-Francoise;Ploton, Dominique
利用免疫荧光共聚焦显微镜和免疫电镜研究了核仁磷酸化蛋白Nopp140在哺乳动物细胞中的分布。在间期，共聚焦切片的三维图像重建显示核仁标记出现在项链状的几个微小球体中。此外，在免疫金标记程序后，在未处理和放线菌素d处理的细胞中，不仅在致密的纤维成分上检测到金颗粒，而且在核仁的纤维中心上也检测到金颗粒。在卡哈尔人的身体中也一直存在标记。用BrUTP进行脉冲追逐实验后，10-15分钟的追逐比5分钟的追逐更明显。在有丝分裂过程中，共聚焦分析表明Nopp140组织丢失。蛋白质在前期分散在染色体之间和周围。从前期中期到末期，在许多细胞质核仁来源的灶中也检测到它。在末期，它再次出现在子核的重组核仁中。这强烈表明Nopp140可能是rDNA转录活性位点参与pre-rRNA加工的一个成分。
We investigated distribution of the nucleolar phosphoprotein Nopp140 within mammalian cells, by immunofluorescence confocal microscopy and immunoelectron microscopy. During interphase, three-dimensional image reconstructions of confocal sections revealed that nucleolar labelling appeared as several tiny spheres organized in necklaces. Moreover, after an immunogold labelling procedure, gold particles were detected not only over the dense fibrillar component but also over the fibrillar centers of nucleoli in untreated and actinomycin D-treated cells. Labelling was also consistently present in Cajal bodies. After pulse-chase experiments with BrUTP, colocalization was more prominent after a 10-15 min chase than after a 5 min chase. During mitosis, confocal analysis indicated that Nopp140 organization was lost. The protein dispersed between and around the chromosomes in prophase. From prometaphase to telophase, it was also detected in numerous cytoplasmic nucleolus-derived foci. During telophase, it reappeared in the reforming nucleoli of daughter nuclei. This strongly suggests that Nopp140 could be a component implicated in pre-rRNA processing by the rDNA transcription active sites.
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NOPP140充当核仁与盘绕体之间的分子联系。
DOI：
10.1083/jcb.142.2.319
发表时间：
1998-07-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Isaac C;Yang Y;Meier UT
通讯作者：
Meier UT
影响因子：
5.6
作者：
Hughes, JMX
通讯作者：
Hughes, JMX
影响因子：
3.3
作者：
JIMENEZGARCIA, LF;SEGURAVALDEZ, MD;SPECTOR, DL
通讯作者：
SPECTOR, DL
DOI：
10.1083/jcb.150.3.433
发表时间：
2000-08-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dundr M;Misteli T;Olson MO
通讯作者：
Olson MO
影响因子：
10.5
作者：
Lafontaine, DLJ;Bousquet-Antonelli, C;Tollervey, D
通讯作者：
Tollervey, D