Differential membrane proteomics using 18O-labeling to identify biomarkers for cholangiocarcinoma.
Differential membrane proteomics using 18O-labeling to identify biomarkers for cholangiocarcinoma.
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DOI：
10.1021/pr800215n
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发表时间：
2008-11
影响因子：
4.4
通讯作者：
Pandey, Akhilesh
中科院分区：
文献类型：
--
作者：
Kristiansen, Troels Zakarias;Harsha, H. C.;Gronborg, Mads;Maitra, Anirban;Pandey, Akhilesh
关键词：
定量蛋白质组学方法允许以高通量方式分析数百至数千种蛋白质。这种方法越来越多地应用于癌症生物标志物的发现，以确定在癌症中差异调节的蛋白质。在质谱分析之前基于细胞亚蛋白质组的富集的蛋白质样品的分级可以提供某些类别分子的增加的覆盖。我们使用了膜蛋白富集策略，结合18O标记的定量蛋白质组学，以确定在胆管癌中高度表达的蛋白质。除了鉴定先前已知在胆管癌中过表达的几种蛋白质外，我们还发现了一些先前与胆管癌无关的分子。使用免疫印迹和免疫组织化学标记的组织芯片，我们验证了高尔基膜蛋白1，膜联蛋白IV和表皮生长因子受体途径底物（Eps8）作为候选生物标志物的胆管癌。高尔基体膜蛋白1在89%的胆管癌组织中表达过强。鉴于最近的报告显示，高尔基体膜蛋白1在血清中是可检测的，进一步研究验证这种蛋白质有可能提供一个生物标志物的早期检测胆管癌。
Quantitative proteomic methodologies allow profiling of hundreds to thousands of proteins in a high throughput fashion. This approach is increasingly applied to cancer biomarker discovery to identify proteins that are differentially regulated in cancers. Fractionation of protein samples based on enrichment of cellular subproteomes prior to mass spectrometric analysis can provide increased coverage of certain classes of molecules. We used a membrane protein enrichment strategy coupled with 18O labeling based quantitative proteomics to identify proteins that are highly expressed in cholangiocarcinomas. In addition to identifying several proteins previously known to be overexpressed in cholangiocarcinoma, we discovered a number of molecules that were previously not associated with cholangiocarcinoma. Using immunoblotting and immunohistochemical labeling of tissue microarrays, we validated Golgi membrane protein 1, Annexin IV and Epidermal growth factor receptor pathway substrate (Eps8) as candidate biomarkers for cholangiocarcinomas. Golgi membrane protein 1 was observed to be overexpressed in 89% of cholangiocarcinoma cases analyzed by staining tissue microarrays. In light of recent reports showing that Golgi membrane protein 1 is detectable in serum; further investigation into validation of this protein has the potential to provide a biomarker for early detection of cholangiocarcinomas.
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DOI：
10.1083/jcb.93.1.97
发表时间：
1982-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fujiki Y;Hubbard AL;Fowler S;Lazarow PB
通讯作者：
Lazarow PB
DOI：
10.1016/s1357-2725(02)00064-x
发表时间：
2002-10-01
影响因子：
4
作者：
Di Fiore, PP;Scita, G
通讯作者：
Scita, G
影响因子：
4.8
作者：
Haataja, L;Kaartinen, V;Heisterkamp, N
通讯作者：
Heisterkamp, N
影响因子：
20.3
作者：
Naik, UP;Naik, MU
通讯作者：
Naik, MU
影响因子：
4.6
作者：
Gores, GJ
通讯作者：
Gores, GJ