Toxoplasma gondii infection in stranded St. Lawrence Estuary beluga Delphinapterus leucas in Quebec, Canada.
Toxoplasma gondii infection in stranded St. Lawrence Estuary beluga Delphinapterus leucas in Quebec, Canada.
复制标题
加拿大魁北克省圣劳伦斯河口搁浅的白鲸 Delphinapterus leucas 感染弓形虫。
DOI：
10.3354/dao03262
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发表时间：
2018
影响因子：
1.4
通讯作者：
B. Dixon
中科院分区：
文献类型：
--
作者：
A. Iqbal;L. Measures;S. Lair;B. Dixon
位于加拿大魁北克的圣劳伦斯河口（SLE）白鲸（Delphinapterus leucas）由于19世纪和20世纪的密集狩猎以及随后的人为污染和该地区的人类活动而濒临灭绝。传染病是这一人群死亡的主要原因。据报道，包括白鲸在内的许多海洋哺乳动物物种体内都有弓形虫寄生虫。本研究对34条搁浅的SLE白鲸的55份组织（心和脑）进行了PCR、DNA测序和限制性片段长度多态性分析（RFLP），以确定T.这些白鲸体内的弓形虫在34头试验的白鲸中，44%呈T。通过PCR检测弓形虫，男性感染率高于女性，新生儿和青少年感染率高于成人。分子生物学分析表明，所有T.感染搁浅的SLE白鲸的弓形虫分为基因型II，其在人类中占优势。虽然我们的研究结果表明搁浅白鲸的高患病率是T。根据公布的尸检结果，只有极少数死亡归因于弓形虫病。弓形虫可以引起一系列疾病，包括神经功能缺损，需要更多的数据来研究这种寄生虫对人口恢复的影响。
The St. Lawrence Estuary (SLE) beluga Delphinapterus leucas in Quebec, Canada, is endangered due to intensive hunting in the 19th and 20th centuries and subsequent anthropogenic contamination and human activities in the region. Infectious disease is a primary cause of death in this population. The protozoan parasite Toxoplasma gondii is reported in numerous marine mammal species, including beluga. In the present study, 55 tissue samples (heart and brain) collected from 34 stranded SLE beluga were analysed by PCR followed by DNA sequencing and restriction fragment length polymorphism analysis (RFLP) to determine the PCR prevalence and genotypes of T. gondii in these beluga. Of 34 beluga tested, 44% were positive for T. gondii by PCR, with males having a higher prevalence of infection than females and with more infected neonates and juveniles than adults. Molecular analyses indicated that all T. gondii infecting stranded SLE beluga grouped into genotype II, which predominates in humans. While our results indicate that a high prevalence of stranded beluga are PCR-positive for T. gondii infection, very few deaths are attributed to toxoplasmosis based on published necropsy results. Toxoplasma gondii can cause a range of diseases, including neurological deficits, and more data are needed to investigate this parasite's effect on population recovery.
影响因子：
4
作者：
Miller, M. A.;Miller, W. A.;Grigg, M. E.
通讯作者：
Grigg, M. E.
影响因子：
4
作者：
Khan A;Dubey JP;Su C;Ajioka JW;Rosenthal BM;Sibley LD
通讯作者：
Sibley LD