In vivo evaluation of pathogenicity and transmissibility of influenza A(H1N1)pdm09 hemagglutinin receptor binding domain 222 intrahost variants isolated from a single immunocompromised patient.
In vivo evaluation of pathogenicity and transmissibility of influenza A(H1N1)pdm09 hemagglutinin receptor binding domain 222 intrahost variants isolated from a single immunocompromised patient.
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DOI：
10.1016/j.virol.2012.02.018
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发表时间：
2012-06-20
期刊：
影响因子：
3.7
通讯作者：
Taubenberger JK
中科院分区：
文献类型：
--
作者：
Memoli MJ;Bristol T;Proudfoot KE;Davis AS;Dunham EJ;Taubenberger JK
甲型H1N1流感pdm09病毒已在全球范围内传播，并继续造成复杂的感染和死亡。报告已经确定了从遭受这种严重感染的个体中分离的病毒中血凝素受体结合域D222G突变的患病率增加，但这种关联仍不清楚。在诊断时，从一名免疫功能低下的重症患者的鼻咽冲洗液中分离出的病毒含有D222，但后来在他的病程中从支气管肺泡灌洗液中收集的分离物主要含有G222突变，并与少数D222群体混合。在雪貂模型中将这些临床分离株与G222空斑纯化病毒进行比较。G222主要临床分离株是雪貂中致病性最强的，在感染过程中在222个氨基酸位置处产生了最多的多样性，这表明HA 222处的多样性增加而不是特异性多态性可能在预测致病潜力方面很重要。
The influenza A(H1N1)pdm09 virus has circulated worldwide and continued to cause complicated infections and deaths. Reports have identified an increased prevalence of the hemagglutinin receptor binding domain D222G mutation in viruses isolated from individuals who have suffered such severe infections, but this association is still unclear. Virus isolated from a nasopharyngeal wash of a severely ill immunocompromised patient at the time of diagnosis contained the D222, but isolates collected later in his course from a bronchoalveolar lavage contained primarily the G222 mutation and was mixed with a minor population of D222. These clinical isolates were compared to a G222 plaque purified virus in the ferret model. The G222 predominant clinical isolate was the most pathogenic in ferrets and developed the most diversity at the 222 amino acid position during infection, suggesting that increased diversity and not a specific polymorphism at HA 222 may be important in predicting pathogenic potential.
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