Genetic characterization of the influenza A pandemic (H1N1) 2009 virus isolates from India.
Genetic characterization of the influenza A pandemic (H1N1) 2009 virus isolates from India.
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DOI：
10.1371/journal.pone.0009693
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发表时间：
2010-03-15
期刊：
影响因子：
3.7
通讯作者：
Mishra AC
中科院分区：
文献类型：
--
作者：
Potdar VA;Chadha MS;Jadhav SM;Mullick J;Cherian SS;Mishra AC
2009年5月，印度出现了甲型流感大流行H1N1 2009（H1N1pdm）病毒，此后该国许多地区报告了具有相当高发病率和死亡率的疫情。持续监测病毒的基因组成对于了解其在国内与全球多样化的关系以及跟踪可能影响病毒行为的突变至关重要。H1N1pdm病毒从代表主要城市的痊愈和死亡病例中分离出来，并进行了测序。参考截至2009年11月24日的685个全球分离株全基因组，对2009年5月至9月的另外7个分离株的6个串联全基因组和血凝素（HA）基因进行了系统发育分析。对所有8个片段的已知致病性标记进行分子表征。2009年5月的第一个分离物属于进化枝5。虽然进化枝7是印度的优势H1N1pdm谱系，但发现进化枝6和7都是共同循环的。所有印度分离株的神经氨酸酶都具有H275，这是神经氨酸酶抑制剂奥司他韦敏感性的标志。HA中的一些突变位于抗原位点处或抗原位点附近，因此可能具有抗原意义。其中，在从致死性病例中获得的8株印度分离株中，发现了2株HA受体结合结构域中的D222G突变。13个印度分离株中的大多数属于全球最广泛传播的H1N1pdm进化枝7。此外，进化枝7印度分离株特有的突变与该国病毒适应性和适应性的相关性仍有待了解。来自致死性病例分离株的HA中的D222G突变需要研究致病性。
The Influenza A pandemic H1N1 2009 (H1N1pdm) virus appeared in India in May 2009 and thereafter outbreaks with considerable morbidity and mortality have been reported from many parts of the country. Continuous monitoring of the genetic makeup of the virus is essential to understand its evolution within the country in relation to global diversification and to track the mutations that may affect the behavior of the virus. H1N1pdm viruses were isolated from both recovered and fatal cases representing major cities and sequenced. Phylogenetic analyses of six concatenated whole genomes and the hemagglutinin (HA) gene of seven more isolates from May-September 2009 was performed with reference to 685 whole genomes of global isolates available as of November 24, 2009. Molecular characterization of all the 8 segments was carried out for known pathogenic markers. The first isolate of May 2009 belonged to clade 5. Although clade 7 was the dominant H1N1pdm lineage in India, both clades 6 and 7 were found to be co-circulating. The neuraminidase of all the Indian isolates possessed H275, the marker for sensitivity to the neuraminidase inhibitor Oseltamivir. Some of the mutations in HA are at or in the vicinity of antigenic sites and may therefore be of possible antigenic significance. Among these a D222G mutation in the HA receptor binding domain was found in two of the eight Indian isolates obtained from fatal cases. The majority of the 13 Indian isolates grouped in the globally most widely circulating H1N1pdm clade 7. Further, correlations of the mutations specific to clade 7 Indian isolates to viral fitness and adaptability in the country remains to be understood. The D222G mutation in HA from isolates of fatal cases needs to be studied for pathogenicity.
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DOI：
10.1073/pnas.85.2.324
发表时间：
1988-01-01
影响因子：
11.1
作者：
KAWAOKA, Y;WEBSTER, RG
通讯作者：
WEBSTER, RG
影响因子：
3.7
作者：
Chakrabarti AK;Pawar SD;Cherian SS;Koratkar SS;Jadhav SM;Pal B;Raut S;Thite V;Kode SS;Keng SS;Payyapilly BJ;Mullick J;Mishra AC
通讯作者：
Mishra AC
影响因子：
64.8
作者：
Neumann, Gabriele;Noda, Takeshi;Kawaoka, Yoshihiro
通讯作者：
Kawaoka, Yoshihiro
影响因子：
64.5
作者：
CATON, AJ;BROWNLEE, GG;GERHARD, W
通讯作者：
GERHARD, W
影响因子：
11.8
作者：
Chen GW;Shih SR
通讯作者：
Shih SR