Structural alterations of the hippocampal formation of adrenalectomized rats: an unbiased stereological study
Structural alterations of the hippocampal formation of adrenalectomized rats: an unbiased stereological study
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肾上腺切除大鼠海马结构的改变：一项无偏见的体视学研究
DOI：
10.1023/a:1018573105987
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
M. Paula
中科院分区：
文献类型：
--
作者：
N. Sousa;M. Madeira;M. Paula
先前的研究已经证明肾上腺切除会迅速诱导海马结构的细胞死亡。然而，这些先前的研究只涉及定性观察或有偏估计。因此，肾上腺切除术效果的选择性和发生变化的程度仍有争议。目前的工作采用无偏见的体视学工具来检查肾上腺切除术对海马结构中神经元数量和体积的影响。雄性大鼠在180日龄处死前15、30或120天切除肾上腺。使用光学分馏器估计海马结构的体层和门中的神经元总数。根据Cavalieri原理确定海马结构中每个亚部的不同层的体积。颗粒细胞总数逐渐减少，达43%。肾上腺切除的动物表现出齿状回的所有层的体积减少。海马结构的其他区域没有显示出显著的细胞损失或体积减少。此外，齿状回的主要神经元亚群也进行了评估，并减少GABA和神经肽Y免疫反应神经元的总数量的分子和颗粒细胞层的肾上腺切除大鼠被发现。没有观察到数量的变化在门。为了表征神经元变性后胶质细胞的反应，我们估计了齿状回中胶质细胞酸性蛋白免疫反应的细胞总数。虽然没有发现胶质细胞总数的变化，在肾上腺切除组中观察到星形胶质细胞活化的迹象。目前的数据加强了证据，指出皮质类固醇激素在维持齿状回的结构完整性的关键作用。
Previous studies have demonstrated that adrenalectomy rapidly induces cell death in hippocampal formation. However, these previous studies have involved only qualitative observations or biased estimates. Therefore, the selectivity of the effects of adrenalectomy and the magnitude of changes occurring, remain controversial. The present work employed unbiased stereological tools to examine the effects of adrenalectomy on the number of neurons in, and the volume of, the hippocampal formation. Male rats were adrenalectomized 15,30 or 120 days before sacrifice at 180 days of age. The total number of neurons in the somal layers and hilus of the hippocampal formation was estimated using the optical fractionator. The volume of the different layers of each subdivision in the hippocampal formation was determined according to the Cavalieri principle. A progressive reduction, reaching 43%, was found in the total number of granule cells. Adrenalectomized animals exhibited a reduction in the volume of all layers of the dentate gyrus. No other region of the hippocampal formation displayed significant cell loss or a reduction in volume. In addition, the main neuronal subpopulations of the dentate gyrus were also evaluated, and a reduction in the total number of GABA- and neuropeptide Y-immunoreactive neurons in the molecular and granule cell layers of adrenalectomized rats was found. No quantitative changes were observed in the hilus. To characterize the glial response to the neuronal degeneration, we estimated the total number of cells immunoreactive for glial fibrillary acidic protein in the dentate gyrus. Although no variation in the total number of glial cells was found, signs of astroglial activation were observed in the adrenalectomized group. The present data strengthen the evidence pointing to the critical role of corticosteroids in maintaining the structural integrity of the dentate gyrus.
影响因子：
56.9
作者：
R. S. Sloviter;G. Valiquette;G. Abrams;Edward C. Ronk;Anne L. Sollas;L. Paul;S. Neubort
通讯作者：
R. S. Sloviter;G. Valiquette;G. Abrams;Edward C. Ronk;Anne L. Sollas;L. Paul;S. Neubort
肾上腺类固醇影响海马神经元的存活。
影响因子：
5.8
作者：
McEwen,BS;Gould,E
通讯作者：
Gould,E