Characterization of the Dynamic Behavior of Neutrophils Following Influenza Vaccination
Characterization of the Dynamic Behavior of Neutrophils Following Influenza Vaccination
复制标题
流感疫苗接种后中性粒细胞动态行为的表征
DOI：
10.3389/fimmu.2019.02621
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发表时间：
2019
影响因子：
7.3
通讯作者：
S. F. Gonzalez
中科院分区：
文献类型：
--
作者：
D. U. Pizzagalli;I. Latino;Alain Pulfer;Miguel Palomino;Tommaso Virgilio;Yagmur Farsakoglu;R. Krause;S. F. Gonzalez
中性粒细胞是最早对炎症和感染做出反应的细胞之一。尽管它们在限制病原体传播方面发挥了关键作用，但对它们在免疫器官中的动态行为的研究仍然难以捉摸。在这项工作中，我们在体内表征了紫外线灭活病毒接种流感疫苗后小鼠延髓淋巴结(PLN)中性粒细胞的动态行为。为了实现这一点，我们使用了一种基于图像的系统生物学方法来检测中性粒细胞的运动模式，并将它们与不同的行为联系起来。我们描述了免疫后中性粒细胞显著和快速地募集到PLN，这依赖于趋化因子CXCL1和ALAMIN分子IL-1α的分泌。此外，我们观察到最初的募集主要是通过位于PLN皮质旁和滤泡间区的高内皮微静脉进行的。中性粒细胞迁移的时空模式分析表明，在最初阶段，大多数中性粒细胞表现为巡视行为，随后在PLN的被膜下窦内形成成群的中性粒细胞，与该室内的巨噬细胞有关。最后，我们使用多种成像技术观察到，中性粒细胞吞噬和运输流感病毒颗粒。这些过程可能对这些细胞呈递病毒抗原的能力有重要影响。
Neutrophils are amongst the first cells to respond to inflammation and infection. Although they play a key role in limiting the dissemination of pathogens, the study of their dynamic behavior in immune organs remains elusive. In this work, we characterized in vivo the dynamic behavior of neutrophils in the mouse popliteal lymph node (PLN) after influenza vaccination with UV-inactivated virus. To achieve this, we used an image-based systems biology approach to detect the motility patterns of neutrophils and to associate them to distinct actions. We described a prominent and rapid recruitment of neutrophils to the PLN following vaccination, which was dependent on the secretion of the chemokine CXCL1 and the alarmin molecule IL-1α. In addition, we observed that the initial recruitment occurred mainly via high endothelial venules located in the paracortical and interfollicular regions of the PLN. The analysis of the spatial-temporal patterns of neutrophil migration demonstrated that, in the initial stage, the majority of neutrophils displayed a patrolling behavior, followed by the formation of swarms in the subcapsular sinus of the PLN, which were associated with macrophages in this compartment. Finally, we observed using multiple imaging techniques, that neutrophils phagocytize and transport influenza virus particles. These processes might have important implications in the capacity of these cells to present viral antigens.
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DOI：
10.1146/annurev-pathol-020712-164023
发表时间：
2014
期刊：
Annual review of pathology
影响因子：
--
作者：
Mayadas TN;Cullere X;Lowell CA
通讯作者：
Lowell CA
感染期间淋巴结中中性粒细胞迁移的动态。
影响因子：
32.4
作者：
Chtanova, Tatyana;Schaeffer, Marie;Han, Seong-Ji;van Dooren, Giel G.;Nollmann, Marcelo;Herzmark, Paul;Chan, Shiao Wei;Satija, Harshita;Camfield, Kristin;Aaron, Holly;Striepen, Boris;Robey, Ellen A.
通讯作者：
Robey, Ellen A.
影响因子：
32.4
作者：
Hou, Baidong;Reizis, Boris;DeFranco, Anthony L.
通讯作者：
DeFranco, Anthony L.
影响因子：
20.3
作者：
Eash, Kyle J.;Means, Jacquelyn M.;Link, Daniel C.
通讯作者：
Link, Daniel C.
影响因子：
32.4
作者：
Miller, LS;O'Connell, RM;Modlin, RL
通讯作者：
Modlin, RL