Subpopulations of dermal skin fibroblasts secrete distinct extracellular matrix: implications for using skin substitutes in the clinic.
Subpopulations of dermal skin fibroblasts secrete distinct extracellular matrix: implications for using skin substitutes in the clinic.
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DOI：
10.1111/bjd.16255
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Higgins CA
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文献类型：
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作者：
Ghetti M;Topouzi H;Theocharidis G;Papa V;Williams G;Bondioli E;Cenacchi G;Connelly JT;Higgins CA
虽然几种商业真皮表皮支架可以促进皮肤的伤口愈合，但实现完全皮肤再生仍然是一个重大挑战。对人皮肤中发现的三种不同成纤维细胞亚群（乳头成纤维细胞（Pfi）、网状成纤维细胞（Rfi）和真皮乳头成纤维细胞（DPfi））的自组装细胞外基质（ECM）进行生物学表征。将成纤维细胞亚群与抗坏血酸一起培养10天，以促进细胞组装的基质产生。随后，去除细胞并表征剩余基质。此外，在另一个实验中，将角质形成细胞接种在细胞耗尽的ECM的顶部以产生仅表皮的皮肤构建体。我们发现，Pfi自组装的ECM表现出与最高原纤维间空间相关的随机取向的纤维，反映了乳头状真皮内生理存在的ECM特征。质谱分析，然后用免疫荧光分析进行验证，表明血小板反应蛋白1优先在DPfi衍生基质中表达。此外，我们观察到在DPfi或Pfi基质上生长的表皮构建体表现出正常的基底膜形成，而Rfi基质不能支持膜形成。我们认为，灵感可以从这些不同的ECM，以改善皮肤工程应用中的治疗性生物材料的设计。 关于这个话题我们已经知道了什么？ 真皮内有几种类型的皮肤成纤维细胞，可以通过它们的空间位置来定义：乳头成纤维细胞（Pfi）、网状成纤维细胞（Rfi）和真皮乳头成纤维细胞（DPfi）。细胞外基质（ECM）的组成是不同的组成和结构的乳头状，网状和毛囊真皮在体内。当皮肤受伤时，用于组织修复的真皮替代物不能反映皮肤真皮内观察到的异质性。 这项研究增加了什么？ 可以在体外产生来自皮肤成纤维细胞的不同亚群的自组装ECM。由Pfi、Rfi和DPfi体外制备的细胞组装ECM反映了体内观察到的真皮异质性，并且在形态、功能和组成上彼此不同。应该从来自不同成纤维细胞亚群的细胞组装ECM中汲取灵感，以改善皮肤工程应用中治疗性生物材料的设计。 翻译的信息是什么？ 来自DPfi和Pfi而不是Rfi的细胞组装ECM可以支持体外邻近角质形成细胞中基底膜的形成。应该从来自不同成纤维细胞亚群的细胞组装ECM中汲取灵感，以改善皮肤工程应用中治疗性生物材料的设计。 链接评论：https://doi.org/10.1111/bjd.16773。 https://doi.org/10.1111/bjd.16946 https://goo.gl/Uqv3dl在线提供
While several commercial dermoepidermal scaffolds can promote wound healing of the skin, the achievement of complete skin regeneration still represents a major challenge. To perform biological characterization of self‐assembled extracellular matrices (ECMs) from three different subpopulations of fibroblasts found in human skin: papillary fibroblasts (Pfi), reticular fibroblasts (Rfi) and dermal papilla fibroblasts (DPfi). Fibroblast subpopulations were cultured with ascorbic acid to promote cell‐assembled matrix production for 10 days. Subsequently, cells were removed and the remaining matrices characterized. Additionally, in another experiment, keratinocytes were seeded on the top of cell‐depleted ECMs to generate epidermal‐only skin constructs. We found that the ECM self‐assembled by Pfi exhibited randomly oriented fibres associated with the highest interfibrillar space, reflecting ECM characteristics that are physiologically present within the papillary dermis. Mass spectrometry followed by validation with immunofluorescence analysis showed that thrombospondin 1 is preferentially expressed within the DPfi‐derived matrix. Moreover, we observed that epidermal constructs grown on DPfi or Pfi matrices exhibited normal basement membrane formation, whereas Rfi matrices were unable to support membrane formation. We argue that inspiration can be taken from these different ECMs, to improve the design of therapeutic biomaterials in skin engineering applications. What's already known about this topic? There are several types of skin fibroblasts within the dermis that can be defined by their spatial location: papillary fibroblasts (Pfi), reticular fibroblasts (Rfi) and dermal papilla fibroblasts (DPfi). Extracellular matrix (ECM) composition is distinct with regard to composition and architecture within the papillary, reticular and hair follicle dermis in vivo. When skin is injured, dermal replacement substitutes used for tissue repair do not reflect the heterogeneity observed within the skin dermis. What does this study add? Self‐assembled ECMs from different subpopulations of skin fibroblasts can be generated in vitro. Cell‐assembled ECMs made in vitro from Pfi, Rfi and DPfi reflect dermal heterogeneity seen in vivo and are morphologically, functionally and compositionally distinct from one another. Inspiration should be taken from cell‐assembled ECMs from distinct fibroblast subpopulations, to improve the design of therapeutic biomaterials in skin engineering applications. What is the translational message? Cell‐assembled ECMs from DPfi and Pfi, but not Rfi, can support formation of a basement membrane in adjacent keratinocytes in vitro. Inspiration should be taken from cell‐assembled ECMs from distinct fibroblast subpopulations, to improve the design of therapeutic biomaterials in skin engineering applications. Linked Comment: https://doi.org/10.1111/bjd.16773. https://doi.org/10.1111/bjd.16946 available online https://goo.gl/Uqv3dl
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影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
影响因子：
3.6
作者：
Frame, JD;Still, J;Burd, A
通讯作者：
Burd, A
影响因子：
6.5
作者：
Janson, David G.;Saintigny, Gaelle;El Ghalbzouri, Abdoelwaheb
通讯作者：
El Ghalbzouri, Abdoelwaheb
DOI：
10.1126/science.1176009
发表时间：
2009-11-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hynes RO
通讯作者：
Hynes RO
影响因子：
64.8
作者：
通讯作者：
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