Phloem unloading in cultivated melon fruits follows an apoplasmic pathway during enlargement and ripening.
Phloem unloading in cultivated melon fruits follows an apoplasmic pathway during enlargement and ripening.
复制标题
DOI：
10.1093/hr/uhad123
复制
发表时间：
2023-08
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
甜瓜(Cucumis Melo L.)在世界范围内有着悠久的种植历史。在栽培、驯化和选育过程中，成熟甜瓜果实的含糖量显著提高。与甜瓜和野生甜瓜相比，甜瓜果实发育中后期蔗糖快速积累。甜瓜果实进化发育过程中的韧皮部卸载途径尚未得到识别和分析。本研究利用CFDA[5(6)-二乙酸碳荧光素]和七叶皂甙示踪、细胞学途径观察、qRT-PCR、基因功能分析等方法对栽培甜瓜和野生甜瓜韧皮部卸载途径及其相关糖转运体的功能进行了分析，结果表明，野生甜瓜韧皮部卸荷途径主要为共生体，而栽培甜瓜果实发育过程中韧皮部卸荷途径由共生体向质外体转移。根据果实嫁接实验，果实汇独立积累糖分。相关分析表明，几个蔗糖转运蛋白基因的表达量与甜瓜果实的蔗糖含量呈正相关。此外，CmSWEET10是一种位于韧皮部质膜上的蔗糖转运蛋白，在甜瓜果实的早熟阶段高表达，这意味着它可能参与了甜瓜果实韧皮质外体的卸载和蔗糖的积累。最后，我们总结了甜瓜果实膨大和蔗糖积累过程中与质外体卸载相关的酶和糖转运蛋白的功能模型。
Melon (Cucumis melo L.) has a long history of cultivation worldwide. During cultivation, domestication, and selection breeding, the sugar content of mature melon fruits has been significantly increased. Compared with unsweet melon and wild melon, rapid sucrose accumulation can occur in the middle and late stages of sweet melon fruit development. The phloem unloading pathway during the evolution and development of melon fruit has not been identified and analyzed. In this study, the phloem unloading pathway and the function of related sugar transporters in cultivated and wild melon fruits were analyzed by CFDA [5(6)-carbofluorescein diacetate] and esculin tracing, cytological pathway observation, qRT–PCR, and gene function analysis, etc. Results show that the phloem unloading pathway of wild melon fruit is largely symplastic, whereas the phloem unloading pathway of cultivated melon fruit shifts from symplastic to apoplasmic during development. According to a fruit grafting experiment, the fruit sink accumulates sugars independently. Correlation analysis showed that the expression amounts of several sucrose transporter genes were positively correlated with the sucrose content of melon fruit. Furthermore, CmSWEET10 was proved to be a sucrose transporter located on the plasma membrane of the phloem and highly expressed in the premature stage of sweet melon fruits, which means it may be involved in phloem apoplast unloading and sucrose accumulation in sweet melon fruits. Finally, we summarize a functional model of related enzymes and sugar transporters involved in the apoplast unloading of sweet melon fruits during enlargement and sucrose accumulation.
登录
查看更多内容
影响因子：
7.4
作者：
Deng, Jinwu;Yang, Xiyan;Zhang, Xianlong
通讯作者：
Zhang, Xianlong
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
7.4
作者：
LINGLE, SE;DUNLAP, JR
通讯作者：
DUNLAP, JR
对中国白梨 (Pyrus bretschneideri) 中甜转运蛋白的进化和功能分化的新见解
影响因子：
4.9
作者：
Li, Jiaming;Qin, Mengfan;Wu, Jun
通讯作者：
Wu, Jun
影响因子：
7.4
作者：
HUBBARD, NL;HUBER, SC;PHARR, DM
通讯作者：
PHARR, DM