Analysis of insulin-like growth factor I gene expression in malignancy: evidence for a paracrine role in human breast cancer.-猫眼课题宝

恶性肿瘤中胰岛素样生长因子 I 基因表达的分析：人类乳腺癌中旁分泌作用的证据。

DOI：

10.1210/mend-3-3-509

发表时间：

1989

期刊：

Molecular endocrinology

影响因子：

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

D. Yee;S. Paik;S. Paik;G. Lebovic;G. Lebovic;R. Marcus;R. Marcus;R. Favoni;R. Favoni;K. Cullen;K. Cullen;M. Lippman;M. Lippman;Neal Rosen;N. Rosen

胰岛素样生长因子I （IGF-I）活性已被报道在几种人类癌症中产生。从igf - 1基因转录的rna的鉴定由于在Northern分析中检测到多个杂交带而变得复杂。为了确定这些rna是否从IGF-I基因转录而来，我们使用了一种敏感和特异性的核糖核酸酶（RNAse）对IGF-I的保护试验。我们还利用原位杂交组织化学技术研究了乳腺癌组织中igf - 1的表达。我们在乳腺癌（11例中没有发现）或结肠癌（9例中没有发现）细胞系中未发现igf - 1 mRNA；这两种肿瘤之前都有表达IGF-I mRNA的报道。然而，三分之三的神经上皮瘤和两分之一的尤文氏肉瘤细胞系表达IGF-I mRNA；因此，在这些肿瘤中，igf - 1可能是一种自分泌生长因子。与乳腺癌细胞系相比，从乳腺组织中提取的RNA很容易检测到IGF-I mRNA。原位杂交显示IGF-I mRNA在基质细胞中表达，而在正常或恶性上皮细胞中不表达。这些发现表明，虽然igf - 1不是由乳腺上皮细胞产生的，但它可能作为上皮细胞的旁分泌刺激剂或基质细胞的自分泌刺激剂起作用。

Insulin-like growth factor I (IGF-I) activity has been reported to be produced by several human cancers. Identification of RNAs transcribed from the IGF-I gene has been complicated by the detection of multiple hybridizing bands on Northern analysis. To determine if any of these RNAs are transcribed from the IGF-I gene, we have used a sensitive and specific ribonuclease (RNAse) protection assay for IGF-I. We have also studied the breast cancer tissue expression of IGF-I using in situ hybridization histochemistry. We have found no IGF-I mRNA in breast (zero of 11) or colon cancer (zero of 9) cell lines; both of these tumors have been previously reported to express IGF-I mRNA. However, three of three neuroepithelioma and one of two Ewing's sarcoma cell lines express IGF-I mRNA; therefore, in these tumors IGF-I may be an autocrine growth factor. In contrast to breast cancer cell lines, RNA extracted from breast tissues has easily detectable IGF-I mRNA. In situ hybridizations show that IGF-I mRNA is expressed in the stromal cells, and not by normal or malignant epithelial cells. These findings suggest that although IGF-I is not produced by breast epithelial cells it may function as either a paracrine stimulator of epithelial cells or an autocrine stimulator of stromal cells.