Ultrastructural, enzyme‐, lectin‐, and immunohistochemical studies of the erosion zone in rat tibiae
Ultrastructural, enzyme‐, lectin‐, and immunohistochemical studies of the erosion zone in rat tibiae
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大鼠胫骨糜烂区的超微结构、酶、凝集素和免疫组织化学研究
DOI：
--
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发表时间：
1996
影响因子：
6.2
通讯作者：
H. Ozawa
中科院分区：
文献类型：
--
作者：
H. Nakamura;H. Ozawa
为了阐明软骨内骨化的过程，我们使用超微结构、酶、凝集素和免疫组织化学技术来研究位于大鼠胫骨侵蚀区的血管周围细胞。在生长板侵蚀区，观察到与毛细血管直接相连的血管周围细胞侵入软骨。这些细胞的细胞质中含有发育良好的粗面内质网和高尔基体，并向未钙化的横软骨壁形成手指状细胞质突起。人们发现这些过程一直延伸到位于生长板钙化层中的退化软骨细胞。有趣的是，这些血管周围细胞既没有表现出碱性磷酸酶活性，也没有表现出耐酒石酸盐的酸性磷酸酶活性。凝集素组织化学显示双花豆凝集素 (DBA) 对血管周围细胞的特异性染色。在内皮细胞、成骨细胞、破软骨细胞或破骨细胞上均未检测到 DBA 反应性。此外，免疫组织化学研究表明，血管周围细胞既不表达CD44，CD44位于破软骨细胞、破骨细胞和骨细胞的质膜上，也不被层粘连蛋白包围。这些结果表明，侵蚀区的血管周围细胞与内皮细胞、成骨细胞、破软骨细胞和破骨细胞不同；它们可以吸收未钙化的软骨基质和退化的软骨细胞；血管周围细胞可能在软骨内骨化过程中的毛细血管侵袭中发挥重要作用。
To clarify the process of endochondral ossification, we used ultrastructural, enzyme‐, lectin‐, and immunohistochemical techniques to study perivascular cells located in the erosion zones of rat tibiae. In growth plate erosion zones, perivascular cells directly connected to blood capillaries were seen invading cartilage. These cells contained a well‐developed rough endoplasmic reticulum and Golgi apparatus in their cytoplasm and formed finger‐like cytoplasmic processes toward uncalcified transverse cartilage walls. These processes were seen to stretch as far as the degenerated chondrocytes located in the calcified layer of the growth plate. Interestingly, these perivascular cells showed neither alkaline phosphatase activity nor tartrate‐resistant acid phosphatase activity. Lectin histochemistry revealed specific staining by Dolichos Biflorus agglutinin (DBA) on the perivascular cells. No reactivity for DBA was detected on either endothelial cells, osteoblasts, chondroclasts, or osteoclasts. In addition, immunohistochemical studies showed that the perivascular cells neither expressed CD44, which was localized on the plasma membrane of chondroclasts, osteoclasts, and osteocytes, nor were surrounded by laminin. These results suggest that the perivascular cells in the erosion zone are distinct from endothelial cells, osteoblasts, chondroclasts, and osteoclasts; that they may resorb uncalcified cartilage matrix and degenerated chondrocytes; and that perivascular cells may play an important role in the capillary invasion during the process of endochondral ossification.
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软骨源性生长因子的纯化。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Klagsbrun,M;Smith,S
通讯作者：
Smith,S
通过肝素亲和层析纯化软骨源性生长因子。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sullivan,R;Klagsbrun,M
通讯作者：
Klagsbrun,M
影响因子：
4.2
作者：
C. Brighton;D. Lorich;R. Kupcha;T. Reilly;A. R. Jones;R. A. Woodbury
通讯作者：
C. Brighton;D. Lorich;R. Kupcha;T. Reilly;A. R. Jones;R. A. Woodbury
透明质酸在骨骺生长板中的分布：表达 CD44 的骨祖细胞的周转。
影响因子：
4
作者：
Pavasant,P;Shizari,TM;Underhill,CB
通讯作者：
Underhill,CB
影响因子：
15.9
作者：
WATSON, KE;BOSTROM, K;DEMER, LL
通讯作者：
DEMER, LL