Genome-wide scan of gastrointestinal nematode resistance in closed Angus population selected for minimized influence of MHC.
Genome-wide scan of gastrointestinal nematode resistance in closed Angus population selected for minimized influence of MHC.
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DOI：
10.1371/journal.pone.0119380
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Van Tassell CP
中科院分区：
文献类型：
--
作者：
Kim ES;Sonstegard TS;da Silva MV;Gasbarre LC;Van Tassell CP
与寄生虫指示性状相关的遗传标记是研究标记辅助选择的理想目标，其目的是控制感染，减少蚁群使用。在本研究中，我们收集了安格斯资源种群断奶后动物的胃肠道（GI）线虫粪卵计数（FEC）数据，这些动物在牧场自然暴露26周。总的来说，在1992年至2007年的16年间收集了487只动物的数据，其中大多数动物被选择为特定的DRB1等位基因，以减少MHC位点潜在等位基因变异效应的影响。基于BovineSNP50基因型的全基因组关联研究（GWAS）揭示了牛染色体3、5、8、15和27上的6个基因组区域；与Box-Cox转换平均FEC （BC-MFEC）显著相关（-log10 p=4.3）。对重要基因组区域内基因的DAVID分析表明，我们的结果与参与感染炎症反应的基因注释之间存在相关性。此外，ROH和选择特征分析提供了强有力的证据，表明与BC-MFEC相关的基因组区域不受局部自合子或最近的实验选择的影响。这些发现为预测年轻放牧牛的寄生虫耐药性提供了有用的信息，并为开发疾病修饰疗法或进一步研究宿主对胃肠道寄生虫感染的反应提供了新的候选基因靶点。
Genetic markers associated with parasite indicator traits are ideal targets for study of marker assisted selection aimed at controlling infections that reduce herd use of anthelminthics. For this study, we collected gastrointestinal (GI) nematode fecal egg count (FEC) data from post-weaning animals of an Angus resource population challenged to a 26 week natural exposure on pasture. In all, data from 487 animals was collected over a 16 year period between 1992 and 2007, most of which were selected for a specific DRB1 allele to reduce the influence of potential allelic variant effects of the MHC locus. A genome-wide association study (GWAS) based on BovineSNP50 genotypes revealed six genomic regions located on bovine Chromosomes 3, 5, 8, 15 and 27; which were significantly associated (-log10 p=4.3) with Box-Cox transformed mean FEC (BC-MFEC). DAVID analysis of the genes within the significant genomic regions suggested a correlation between our results and annotation for genes involved in inflammatory response to infection. Furthermore, ROH and selection signature analyses provided strong evidence that the genomic regions associated BC-MFEC have not been affected by local autozygosity or recent experimental selection. These findings provide useful information for parasite resistance prediction for young grazing cattle and suggest new candidate gene targets for development of disease-modifying therapies or future studies of host response to GI parasite infection.
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影响因子：
3.3
作者：
Keller MC;Visscher PM;Goddard ME
通讯作者：
Goddard ME
影响因子：
4
作者：
Ingham, Aaron;Reverter, Antonio;Menzies, Moira
通讯作者：
Menzies, Moira
影响因子：
14.9
作者：
Fujita PA;Rhead B;Zweig AS;Hinrichs AS;Karolchik D;Cline MS;Goldman M;Barber GP;Clawson H;Coelho A;Diekhans M;Dreszer TR;Giardine BM;Harte RA;Hillman-Jackson J;Hsu F;Kirkup V;Kuhn RM;Learned K;Li CH;Meyer LR;Pohl A;Raney BJ;Rosenbloom KR;Smith KE;Haussler D;Kent WJ
通讯作者：
Kent WJ
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.
影响因子：
2.6
作者：
Gasbarre, Louis C.
通讯作者：
Gasbarre, Louis C.