Highly sensitive Curcumin-conjugated nanotheranostic platform for detecting amyloid-beta plaques by magnetic resonance imaging and reversing cognitive deficits of Alzheimer's disease via NLRP3-inhibition.
Highly sensitive Curcumin-conjugated nanotheranostic platform for detecting amyloid-beta plaques by magnetic resonance imaging and reversing cognitive deficits of Alzheimer's disease via NLRP3-inhibition.
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高灵敏度姜黄素偶联纳米治疗平台，用于通过磁共振成像检测淀粉样蛋白斑块，并通过 NLRP3 抑制逆转阿尔茨海默病的认知缺陷
DOI：
10.1186/s12951-022-01524-4
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发表时间：
2022-07-14
影响因子：
10.2
通讯作者：
Liu, Jun
中科院分区：
文献类型：
--
作者：
Ruan, Yuting;Xiong, Ying;Fang, Wenli;Yu, Qun;Mai, Yingren;Cao, Zhiyu;Wang, Kexi;Lei, Ming;Xu, Jiaxin;Liu, Yan;Zhang, Xingcai;Liao, Wang;Liu, Jun
阿尔茨海默病（AD）是最常见的神经退行性疾病，目前尚无有效的治疗方法，并且缺乏针对临床前 AD 患者的诊断策略。迫切需要发展 AD 的早期诊断和治疗干预。在此，我们开发了一个纳米治疗平台，由抗炎分子姜黄素（Cur）和超顺磁性氧化铁（SPIO）纳米颗粒组成，纳米颗粒由二嵌段1,2-二-leoyl-sn-glycero-3-磷酸乙醇胺-n-[聚（乙二醇）]（DSPE-PEG）封装，表面经过CRT和QSH肽修饰。此外，我们证明这种多功能纳米材料可以有效减少 β-淀粉样蛋白斑块负担，特别是在 APP/PS1 转基因小鼠中，这一过程可通过磁共振成像 (MRI) 无创检测，并将二维 MRI 图像计算成三维 (3D) 绘图。我们的数据证明了对 β-淀粉样蛋白斑块的高度灵敏的体内检测，这比之前报道的更接近地揭示了 Aβ 的真实沉积，并且我们首次基于 3D 图定量了斑块的体积。此外，小鼠的记忆缺陷得到显着改善，这可能与抑制 NLR Family Pyrin Domain Containing 3 (NLRP3) 炎症小体有关。收集的数据表明，该治疗诊断平台可能具有 AD 的早期诊断和治疗潜力。在线版本包含可在 10.1186/s12951-022-01524-4 获取的补充材料。
Alzheimer's disease (AD) is the most common neurodegenerative disorder without effective therapy and lack diagnosis strategy for preclinical AD patients. There is an urgent need for development of both early diagnosis and therapeutic intervention of AD. Herein, we developed a nanotheranostics platform consisting of Curcumin (Cur), an anti-inflammatory molecule, and superparamagnetic iron oxide (SPIO) nanoparticles encapsulated by diblock 1,2-dio-leoyl-sn-glycero-3-phosphoethanolamine-n-[poly(ethylene glycol)] (DSPE-PEG) that are modified with CRT and QSH peptides on its surface. Furthermore, we demonstrated that this multifunctional nanomaterial efficiently reduced β-amyloid plaque burden specifically in APP/PS1 transgenic mice, with the process noninvasively detected by magnetic resonance imaging (MRI) and the two-dimensional MRI images were computed into three-dimension (3D) plot. Our data demonstrated highly sensitive in vivo detection of β-amyloid plaques which more closely revealed real deposition of Aβ than previously reported and we quantified the volumes of plaques for the first time based on 3D plot. In addition, memory deficits of the mice were significantly rescued, probably related to inhibition of NLR Family Pyrin Domain Containing 3 (NLRP3) inflammasomes. Gathered data demonstrated that this theranostic platform may have both early diagnostic and therapeutic potential in AD. The online version contains supplementary material available at 10.1186/s12951-022-01524-4.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
8.8
作者：
Friker LL;Scheiblich H;Hochheiser IV;Brinkschulte R;Riedel D;Latz E;Geyer M;Heneka MT
通讯作者：
Heneka MT
影响因子：
6
作者：
Fahmy AM;El-Setouhy DA;Ibrahim AB;Habib BA;Tayel SA;Bayoumi NA
通讯作者：
Bayoumi NA
影响因子：
14
作者：
Boccafoschi, F;Habermehl, J;Mantovani, D
通讯作者：
Mantovani, D
影响因子：
3.6
作者：
Kannan, Ramalingam G.;Abhilash, Maliakal B.;Krishnakumar, I. M.
通讯作者：
Krishnakumar, I. M.