Bioactive dietary polyphenols inhibit heme iron absorption in a dose-dependent manner in human intestinal Caco-2 cells.
Bioactive dietary polyphenols inhibit heme iron absorption in a dose-dependent manner in human intestinal Caco-2 cells.
复制标题
DOI：
10.1111/j.1750-3841.2011.02184.x
复制
发表时间：
2011-06
影响因子：
3.9
通讯作者：
Han O
中科院分区：
文献类型：
--
作者：
Ma Q;Kim EY;Lindsay EA;Han O
虽然血红素铁是膳食铁的一种重要形式，但其肠道吸收机制仍不清楚。我们以前的工作表明，（−）-表没食子儿茶素-3-没食子酸酯（EGCG）和葡萄籽提取物（GSE）通过减少Caco-2细胞基底外侧铁输出，显著抑制肠血红素铁吸收。本研究的目的是检查是否少量的EGCG，GSE和绿色茶提取物（GT）可以抑制血红素铁的吸收，并测试是否多酚的抑制作用可以抵消抗坏血酸。通过在不存在和存在抗坏血酸的情况下向Caco-2细胞添加不同浓度的EGCG、GSE和GT来进行血红素-55 Fe吸收研究。多酚类化合物显着抑制血红素-55Fe吸收的剂量依赖性方式。当多酚浓度为46 mg/L时，抗坏血酸的加入不能调节膳食多酚对血红素铁吸收的抑制作用。当多酚浓度较低（≤ 4.6mg/L）时，抗坏血酸能够抵消或逆转多酚类化合物的抑制作用。抗坏血酸调节血红素铁的吸收，而不改变顶端血红素摄取，参与血红素代谢和基底侧铁转运的蛋白质的表达，以及血红素加氧酶活性，表明抗坏血酸可能通过调节55 Fe的细胞内分布来增强血红素铁的吸收。这些结果表明，经常食用膳食抗坏血酸可以很容易地抵消低浓度的膳食多酚对血红素铁吸收的抑制作用，但不能抵消高浓度的多酚的抑制作用。
Although heme iron is an important form of dietary iron, its intestinal absorption mechanism remains elusive. Our previous work revealed that (−)-epigallocatechin-3-gallate (EGCG) and grape seed extract (GSE) markedly inhibited intestinal heme iron absorption by reducing the basolateral iron export in Caco-2 cells. The aims of this study were to examine whether small amounts of EGCG, GSE and green tea extract (GT) could inhibit heme iron absorption, and to test whether the inhibitory action of polyphenols could be offset by ascorbic acid. A heme-55Fe absorption study was conducted by adding various concentrations of EGCG, GSE and GT to Caco-2 cells in the absence and presence of ascorbic acid. Polyphenolic compounds significantly inhibited heme-55Fe absorption in a dose-dependent manner. The addition of ascorbic acid did not modulate the inhibitory effect of dietary polyphenols on heme iron absorption when the cells were treated with polyphenols at a concentration of 46 mg/L. However, ascorbic acid was able to offset or reverse the inhibitory effects of polyphenolic compounds when lower concentrations of polyphenols were added (≤ 4.6 mg/L). Ascorbic acid modulated the heme iron absorption without changing the apical heme uptake, the expression of the proteins involved in heme metabolism and basolateral iron transport, and heme oxygenase activity, indicating that ascorbic acid may enhance heme iron absorption by modulating the intracellular distribution of 55Fe. These results imply that the regular consumption of dietary ascorbic acid can easily counteract the inhibitory effects of low concentrations of dietary polyphenols on heme iron absorption but cannot counteract the inhibitory actions of high concentrations of polyphenols.
登录
查看更多内容
影响因子：
4.2
作者：
Kim, Eun-Young;Ham, Soo-Kyung;Han, Okhee
通讯作者：
Han, Okhee
DOI：
10.1093/jn/138.9.1677
发表时间：
2008-09
期刊：
The Journal of nutrition
影响因子：
--
作者：
Bose M;Lambert JD;Ju J;Reuhl KR;Shapses SA;Yang CS
通讯作者：
Yang CS
影响因子：
7.1
作者：
HALLBERG, L;BRUNE, M;ROSSANDER, L
通讯作者：
ROSSANDER, L
影响因子：
4
作者：
Han, Okhee;Kim, Eun-Young
通讯作者：
Kim, Eun-Young
影响因子：
4.2
作者：
Ma, Qianyi;Kim, Eun-Young;Han, Okhee
通讯作者：
Han, Okhee