Insulin-induced formation of ruffling membranes of KB cells and its correlation with enhancement of amino acid transport
Insulin-induced formation of ruffling membranes of KB cells and its correlation with enhancement of amino acid transport
复制标题
胰岛素诱导的KB细胞皱褶膜的形成及其与氨基酸转运增强的相关性
作者：
K. Goshima;A. Masuda;K. Owaribe
在加入胰岛素后1- 2分钟内，在培养的KB细胞（来源于人表皮样癌的细胞株）中诱导褶皱膜的形成。皱褶区域用肌动蛋白抗体而不是微管蛋白抗体强烈染色。KB细胞的预处理剂破坏微丝（细胞松弛素），但不与那些破坏微管（秋水仙胺，nocodazole，秋水仙碱）完全抑制皱褶膜的形成。用双丁酰环腺苷酸预处理KB细胞，但不与双丁酰环鸟苷酸，也抑制皱褶膜的形成。添加胰岛素后5分钟内，细胞对系统A氨基酸（α-氨基异丁酸; AIB）的Na+依赖性摄取增强，并且细胞的环AMP含量降低。抑制胰岛素诱导的KB细胞皱褶膜形成的治疗也抑制胰岛素诱导的AIB摄取增强。从这些观察结果，胰岛素诱导的皱褶膜的形成及其与AIB运输的密切相关性的机制进行了讨论。
Insulin induced the formation of ruffling membranes in cultured KB cells (a cell strain derived from human epidermoid carcinoma) within 1- 2 min after its addition. The ruffled regions were stained strongly with antibody to actin but not that to tubulin. Pretreatment of KB cells with agents disrupting microfilaments (cytochalasins), but not with those disrupting microtubules (colcemid, nocodazole, and colchicine) completely inhibited the formation of ruffling membranes. Pretreatment of KB cells with dibutyryl cyclic AMP, but not with dibutyryl cyclic GMP, also inhibited the formation of ruffling membranes. Addition of insulin enhanced Na+-dependent uptake of a system A amino acid (alpha-amino isobutyric acid; AIB) by the cells within 5 min after the addition, and decreased the cyclic AMP content of the cells. Treatments that inhibited insulin-induced formation of ruffling membranes of KB cells also inhibited insulin-induced enhancement of their AIB uptake. From these observations, the mechanism of insulin-induced formation of ruffling membranes and its close correlation with AIB transport are discussed.
在胶原凝胶/尼龙网上培养的成年大鼠肝细胞中微丝的积累以及氨基酸和葡萄糖的转运。
DOI：
10.1016/0006-291x(81)91889-1
发表时间：
1981
影响因子：
3.1
作者：
Mak,WW;Pitot,HC
通讯作者：
Pitot,HC
有证据表明，葡萄糖转运活性的易位是胰岛素作用于脂肪细胞中葡萄糖转运的主要机制。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kono,T;Robinson,FW;Blevins,TL;Ezaki,O
通讯作者：
Ezaki,O