Transcription in nematodes: early Ascaris embryos are transcriptionally active.

Transcription in nematodes: early Ascaris embryos are transcriptionally active.
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线虫中的转录:早期蛔虫胚胎具有转录活性。

DOI:
10.1016/0012-1606(89)90062-6
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发表时间:
1989
影响因子:
2.7
通讯作者:
Karen L. Bennett
Karen L. Bennett
中科院分区:
生物学3区
文献类型:
--
作者:
Peter J. Cleavinger;James W. McDowell;Karen L. Bennett

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在这份报告中,我们分析了早期合子基因在寄生线虫蛔虫蛔虫。使用早期胚胎阶段的同步群体,分离细胞核,并进行体外转录检测。我们发现转录活性早在4至8细胞阶段。如在这些测定中测量的,RNA聚合酶II活性的百分比在60细胞阶段为总转录的>80%。此外,我们表明,一个特定的转录(肌动蛋白)可以确定在所有的早期阶段测试。
In this report we analyze early zygotic gene expression in the parasitic nematodeAscaris lumbricoidesvar.suum. Using synchronous populations of early embryonic stages, nuclei were isolated, andin vitrorun-off transcription assays were performed. We find transcriptional activity as early as the 4- to 8-cell stage. The percentage of RNA polymerase II activity, as measured in these assays, is >80% of the total transcription at the 60-cell stage. Furthermore, we show that a specific transcript (actin) can be identified in all early stages tested.
来自野生型和 α-鹅膏蕈碱抗性线虫菌株的 RNA 聚合酶 II。
DOI: --
发表时间: 1983
期刊: The Journal of biological chemistry
影响因子: --
作者:
Sanford,T;Golomb,M;Riddle,DL
通讯作者: Riddle,DL
DOI: 10.1016/0022-2836(83)90056-6
发表时间: 1983-01-01
影响因子: 5.6
作者:
FILES, JG;CARR, S;HIRSH, D
通讯作者: HIRSH, D