Evaluation of enhanced high-resolution laser Doppler imaging in an in vitro tube model with the aim of assessing blood flow in separate microvessels.
Evaluation of enhanced high-resolution laser Doppler imaging in an in vitro tube model with the aim of assessing blood flow in separate microvessels.
复制标题
在体外管模型中评估增强型高分辨率激光多普勒成像，旨在评估单独微血管中的血流。
作者：
Maria Lindén;H. Golster;S. Bertuglia;Antonio Colantuoni;Folke Sjöberg;Gert Nilsson
一种增强的高分辨率激光多普勒成像（EHR-LDI）技术旨在通过使用在体外流动模型的可视化单独的微血管进行了评价。在EHR-LDI中，聚焦到半功率直径小于40微米的激光束以25微米的步长连续扫描研究中的组织。1.5 x 1.5 mm的微血管结构内的空间血流变化由64 x 64个测量部位呈现。可以估计单个微血管直径，与显微镜测量值相比，平均差异为11 μ m。对于所使用的流量算法，当用人血灌注内径为175微米的塑料管时，发现LDI输出信号与平均速度（0-3.5 mm/s）成线性比例（相关系数0.99）。还发现LDI输出信号对16- 44%范围内的血细胞比容变化不敏感。由于激光在血液中的穿透有限，当内管直径从280 μ m连续改变到1400 μ m时，观察到LDI输出信号减小。
An enhanced high-resolution laser Doppler imaging (EHR-LDI) technique intended for visualization of separate microvessels was evaluated by use of in vitro flow models. In EHR-LDI, a laser beam focused to a half-power diameter less than 40 microm successively scans the tissue under study in steps of 25 microm. Spatial blood flow variations within microvascular structures of 1.5 x 1.5 mm are rendered by 64 x 64 measurement sites. Individual microvessel diameters could be estimated and an average difference of 11 microm compared to microscopic measurements was obtained. For the flow algorithm used, the LDI output signal was found to scale linearly with average velocity (0-3.5 mm/s) when a plastic tube of inner diameter 175 microm was perfused with human blood (correlation coefficient 0.99). The LDI output signal was further found insensitive to hematocrit variations in the range 16-44%. Due to the limited laser light penetration in blood, a reduction in the LDI output signal was observed as the inner tube diameters were successively changed from 280 to 1400 microm.
影响因子：
4.4
作者：
C. Riva;J. Grunwald;S. Sinclair;B. Petrig
通讯作者：
C. Riva;J. Grunwald;S. Sinclair;B. Petrig
血流量的测量。
影响因子：
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作者：
BermanJr,W;ListerJr,G;Pitt,BR;Hoffman,JI
通讯作者：
Hoffman,JI