Regulation of globin gene expression in human K562 cells by recombinant activin A
Regulation of globin gene expression in human K562 cells by recombinant activin A
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重组激活素 A 调控人 K562 细胞中珠蛋白基因表达
DOI：
10.1182/blood.v79.3.765.bloodjournal793765
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发表时间：
1992
期刊：
影响因子：
20.3
通讯作者：
John Yu
中科院分区：
文献类型：
--
作者：
N. Frigon;L. Shao;A. L. Young;L. Maderazo;John Yu
最近的研究表明，纯化的蛋白质，激活素A，属于转化生长因子β（TGF-β）超家族。与TGF-β类似，激活素A可以具有不同的生物活性，这取决于靶组织。我们使用重组激活素A来证明这种蛋白质在调节人红系细胞系K562中的人红系分化中可能的调节作用。使用基因组探针含有第二外显子的α，β，γ，和α珠蛋白，相对丰度的各种类型的珠蛋白转录物在未经处理和激活素处理的K562细胞进行了检查与S1核酸酶分析。尽管各种珠蛋白序列之间具有相当大的同源性，但这些珠蛋白探针在分析中对其独特的mRNA种类具有高度特异性。结果表明，γ球蛋白和α球蛋白（209个核苷酸）以及α球蛋白（180个核苷酸）的特异性球蛋白探针片段的丰度在用激活素A处理的K562细胞中增加了许多倍，其中γ球蛋白和α球蛋白被保护免于S1消化。相比之下，在对照或激活素处理的细胞中均未检测到β珠蛋白的特异性转录物。胎儿和胚胎β样和α球蛋白转录物水平的增加也证实了早期使用球蛋白cDNA作为探针的北方和狭缝印迹分析的研究。此外，使用分离的细胞核的核径流转录测定表明，激活素处理后珠蛋白转录物的增加大部分可归因于珠蛋白基因转录速率的刺激。瞬时转染试验也提供了证据，激活素A显着刺激转录活性的一个α珠蛋白启动子在K562，但不是在nonerythroid中国仓鼠卵巢细胞。因此，可以得出结论，激活素A在红系细胞中在转录水平上对珠蛋白基因表达发挥其作用。
Recent studies indicate that a purified protein, activin A, belongs to the transforming growth factor beta (TGF-beta) superfamily. Similar to TGF-beta, activin A can have different biologic activities, depending on the target tissues. We used recombinant activin A to demonstrate a possible regulatory role of this protein in modulating human erythroid differentiation in the human erythroid cell line, K562. Using genomic probes containing the second exon of alpha, beta, gamma, and epsilon globins, relative abundance of various types of globin transcripts in untreated and activin-treated K562 cells was examined with S1 nuclease analysis. Despite considerable homology amongst various globin sequences, these globin probes were highly specific for their unique mRNA species in the analyses. It was shown that the abundance of specific globin probe fragments for gamma and epsilon globins (209 nucleotides) as well as alpha (180 nucleotides), which were protected from S1 digestion, increased many fold in K562 cells treated with activin A. In contrast, there were no specific transcripts of beta globin detected in either the control or activin-treated cells. The increases in the level of fetal and embryonic beta-like and alpha globin transcripts also confirmed earlier studies of Northern and slot- blot analyses using globin cDNA as probes. In addition, nuclear run-off transcription assay using isolated nuclei indicated that most of the increase in the globin transcripts after activin treatment could be attributed to the stimulation of transcription rate for globin genes. Transient transfection assays also provide evidence that activin A significantly stimulated transcriptional activity of an epsilon globin promoter in K562, but not in the nonerythroid Chinese hamster ovary cells. Therefore, it was concluded that activin A exerts its effects on globin gene expression at the level of transcription in erythroid cells.
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影响因子：
20.3
作者：
Yu,J;Shao,L;Vaughan,J;Vale,W;Yu,AL
通讯作者：
Yu,AL
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DOI：
10.1126/science.6607531
发表时间：
1984
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hutson,JM;Fallat,ME;Kamagata,S;Donahoe,PK;Budzik,GP
通讯作者：
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重组人粒细胞-巨噬细胞集落刺激因子的体外生物学特性。
影响因子：
20.3
作者：
Metcalf,D;Begley,CG;Johnson,GR;Nicola,NA;Vadas,MA;Lopez,AF;Williamson,DJ;Wong,GG;Clark,SC;Wang,EA
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激活素 A 对纯化的人红系祖细胞中珠蛋白基因表达的影响。
影响因子：
20.3
作者：
Shao,L;FrigonJr,NL;Young,AL;Yu,AL;Mathews,LS;Vaughan,J;Vale,W;Yu,J
通讯作者：
Yu,J
DOI：
10.1073/pnas.81.22.7161
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
POTTER, H;WEIR, L;LEDER, P
通讯作者：
LEDER, P