Survival of an Aggregatibacter actinomycetemcomitans quorum sensing luxS mutant in the mouths of Rhesus monkeys: insights into ecological adaptation.
Survival of an Aggregatibacter actinomycetemcomitans quorum sensing luxS mutant in the mouths of Rhesus monkeys: insights into ecological adaptation.
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DOI：
10.1111/omi.12184
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发表时间：
2017-10
影响因子：
3.7
通讯作者：
Fine DH
中科院分区：
文献类型：
--
作者：
Velusamy SK;Sampathkumar V;Godboley D;Fine DH
Experiments were designed to explore a prominent autoinducer-2 (AI-2) producing gene (luxS) related to colonization and survival of Aggregatibacter actinomycetemcomitans, a low abundance member of the indigenous flora, that forms a key component of the dysbiotic flora in localized aggressive periodontitis. The luxS gene was disrupted in a primate strain of A. actinomycetemcomitans prior to implantation into the oral cavity of Rhesus monkeys (Rh). The colonization efficiency of the luxS mutant (RhAa-VS4) was compared to the parental wild-type strain (RhAa3) (positive control) and a ltxA mutant (RhAa-VS2) (negative control). The in vivo results showed that the luxS mutation had minimal impact on A. actinomycetemcomitans colonization as compared to the wild-type RhAa3 strain. In vitro studies revealed that there was a significant up-regulation of attachment related aae, apiA and flp in the RhAa-VS4 strain as compared to RhAa3. Biofilm forming ability was also significantly increased in RhAa-VS4 strain compared to RhAa3, while the AI-2 signal was ablated. The addition of the AI-2 precursor dihydroxy pentanedione (DPD) allowed the RhAa-VS4 strain to achieve RhAa3 biofilm levels. This is the first primate study to test the relevance of LuxS in vivo. In vitro assessment suggests that survival of RhAa-VS4 strain was due to the production of signaling autoinducer molecules derived from other members of the flora as well as the up-regulation of genes related to attachment and biofilm formation.
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影响因子：
2.9
作者：
Chahboun H;Arnau MM;Herrera D;Sanz M;Ennibi OK
通讯作者：
Ennibi OK
影响因子：
3.2
作者：
Barrios, AFG;Zuo, RJ;Wood, TK
通讯作者：
Wood, TK
DOI：
10.1073/pnas.97.16.8789
发表时间：
2000-08-01
影响因子：
11.1
作者：
Parsek, MR;Greenberg, EP
通讯作者：
Greenberg, EP
影响因子：
3.3
作者：
van der Vinne, AN;Lo, RYC;Shewen, PE
通讯作者：
Shewen, PE
影响因子：
3.7
作者：
Shanmugam M;Gopal P;El Abbar F;Schreiner HC;Kaplan JB;Fine DH;Ramasubbu N
通讯作者：
Ramasubbu N