Virtual tissue staining in pathology using machine learning
Virtual tissue staining in pathology using machine learning
复制标题
使用机器学习进行病理学虚拟组织染色
DOI：
10.1080/14737159.2022.2153040
复制
发表时间：
2022
影响因子：
5.1
通讯作者：
Ozcan, Aydogan
中科院分区：
文献类型：
--
作者：
Pillar, Nir;Ozcan, Aydogan
病理学是一门诊断和研究疾病的医学学科。通过识别结构组织学改变，病理学家获得这些变化对细胞和组织功能的影响的有价值的信息。病理学评估是通过光学显微镜检查安装在玻璃载玻片上的组织学染色组织，或者近年来通过数字化版本的组织学图像（即数字整片成像，WSI）进行的。长期建立的病理工作流程包括一系列制备染色组织样本的过程，包括固定、处理、包埋、切片和染色[1]。染色用于突出组织的重要特征，以及增强组织对比度。由于大多数化学染色试剂的毒性，这通常是一个费时费力的过程，需要由训练有素的技术人员在指定的实验室基础设施中进行。半自动化或手动染色过程以及不同化学试剂的使用导致样品制备的技术差异很大，有时会导致诊断挑战。此外，染色过程使组织扭曲，并禁止对任何特定切片进行额外染色和对同一切片进行进一步的分子分析。这在诊断困难病例的小组织活检中非常重要，因为通常需要多次染色，然后可能需要辅助检测（例如DNA/RNA测序）才能做出诊断。如果所有组织活检材料都用于染色，则无法进行此类分子分析。
Pathology is a medical discipline dealing with diagnosing and studying diseases. Through recognizing structural histological alterations, pathologists acquire valuable information on the effect of these changes on cellular and tissue function. Pathologist evaluation is performed by examination of histologically stained tissue mounted on a glass slide through an optical microscope, or, in recent years, of a digitized version of the histological image (ie digital whole slide imaging, WSI). The long-established pathology workflow consists of a series of processes to prepare stained tissue samples, which involve fixation, processing, embedding, sectioning and staining [1]. Staining is used to highlight important features of the tissue, as well as to enhance tissue contrast. This is in general a time-consuming, laborious process that needs to be performed in a designated lab infrastructure by trained technicians due to the toxicity of most chemical staining reagents. The semi-automated or manual staining processes and the utilization of different chemical reagents lead to high technical variability in sample preparation, which sometimes causes diagnostic challenges. Furthermore, the staining process distorts the tissue and prohibits additional staining of any specific section and further molecular analysis on the same section. This is highly important in small tissue biopsies of diagnostically challenging cases, where multiple stains are often needed, followed by ancillary tests (eg DNA/RNA sequencing) that may be required to reach a diagnosis. If all the tissue biopsy material is used for staining, such molecular analysis cannot be performed.
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DOI：
10.5539/gjhs.v8n3p72
发表时间：
2015-06-25
期刊：
Global journal of health science
影响因子：
--
作者：
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通讯作者：
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影响因子：
--
作者：
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通讯作者：
Ozcan A
DOI：
10.1038/s41377-021-00674-8
发表时间：
2021-11-18
期刊：
Light, science & applications
影响因子：
--
作者：
Li J;Garfinkel J;Zhang X;Wu D;Zhang Y;de Haan K;Wang H;Liu T;Bai B;Rivenson Y;Rubinstein G;Scumpia PO;Ozcan A
通讯作者：
Ozcan A
影响因子：
19.4
作者：
Zhang, Yijie;de Haan, Kevin;Ozcan, Aydogan
通讯作者：
Ozcan, Aydogan
影响因子：
--
作者：
Rivenson Y;de Haan K;Wallace WD;Ozcan A
通讯作者：
Ozcan A