Microsphere-based immunoassay for the detection of azaspiracids.
Microsphere-based immunoassay for the detection of azaspiracids.
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DOI：
10.1016/j.ab.2013.10.035
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发表时间：
2014-02-15
影响因子：
2.9
通讯作者：
Botana LM
中科院分区：
文献类型：
--
作者：
Rodríguez LP;Vilariño N;Louzao MC;Dickerson TJ;Nicolaou KC;Frederick MO;Botana LM
Azaspiracids (AZAs) are a group of lipophilic toxins discovered in mussels from Ireland in 1995 following a human poisoning incident. Nowadays the regulatory limit for AZAs in many countries is set at 160 Fg of azaspiracid equivalents per kg of shellfish meat. In this work a microsphere-based immunoassay has been developed for the detection of AZAs using a Luminex system. This method is based on the competition between AZA-2 immobilized onto the surface of microspheres and free AZAs for the interaction with a monoclonal anti-azaspiracid antibody (mAb 8F4). In this inhibition immunoassay the amount of mAb 8F4 bound to AZA-2-microspheres was quantified using a phycoerythrin-labeled anti-mouse antibody, and the fluorescence was measured with a Luminex analyzer. Simple acetate/methanol or methanol extractions yielded final extracts with no matrix interferences and adequate recovery rates of 86.5% and 75.8%, respectively. In summary, this work presents, a sensitive and easily performed screening method capable of detecting AZAs at concentrations below the range of the European regulatory limit using a microsphere/flow cytometry system.
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影响因子：
7.4
作者：
Fraga, Maria;Vilarino, Natalia;Botana, Luis M.
通讯作者：
Botana, Luis M.
影响因子：
2.8
作者：
Alvarez, Gonzalo;Uribe, Eduardo;Blanco, Juan
通讯作者：
Blanco, Juan
影响因子：
5.4
作者：
Twiner, Michael J.;Rehmann, Nils;Hess, Philipp;Doucette, Gregory J.
通讯作者：
Doucette, Gregory J.
影响因子：
2.8
作者：
James, KJ;Furey, A;Yasumoto, T
通讯作者：
Yasumoto, T
影响因子：
15
作者：
Forsyth, Craig J.;Xu, Jianyan;Miles, Christopher O.
通讯作者：
Miles, Christopher O.