Mouse polo-like kinase 1 associates with the acentriolar spindle poles, meiotic chromosomes and spindle midzone during oocyte maturation
Mouse polo-like kinase 1 associates with the acentriolar spindle poles, meiotic chromosomes and spindle midzone during oocyte maturation
复制标题
小鼠 Polo 样激酶 1 在卵母细胞成熟过程中与无心粒纺锤体极、减数分裂染色体和纺锤体中区相关
作者：
F. Wianny;Á. Tavares;M. Evans;D. Glover;M. Zernicka
抽象的。我们研究了小鼠卵母细胞成熟过程中polo样激酶1（Plk1）的定位动态。Plk1蛋白水平增加后，germinal囊泡破裂，此时酶开始积累在浓缩染色体上的离散位置，随后，在减数分裂纺锤体的两极，向皮质的鸡蛋。有趣的是，在两次减数分裂的中期，Plk 1显示出沿着宽纺锤体两极的点状定位。Plk 1在减数分裂染色体上的点状分布出现在后期早期，与着丝粒区相对应。该蛋白在后期重新定位到纺锤体中间区，然后在末期与中间体结合。我们已经证实了在固定制剂中观察到的免疫定位的特定模式，通过观察在活卵母细胞中标记有绿色荧光蛋白的Plk 1的分布。我们讨论了本地化的酶在光的纺锤杆，这是已知的缺乏中心粒的结构，和高度不对称的性质的减数分裂分裂。
Abstract. We have examined the dynamics of the localisation of the polo-like kinase 1 (Plk1) during maturation of the mouse oocyte. Levels of Plk1 protein increase following germinal vesicle breakdown, at which time the enzyme begins to accumulate at discrete positions on the condensing chromosomes and, subsequently, at the poles of the meiotic spindle, which moves towards the cortex of the egg. Interestingly, at metaphase in both meiotic divisions, Plk1 shows a punctate localisation along the broad spindle poles. Moreover, the punctate distribution of Plk1 on the meiotic chromosomes appears at early anaphase to correspond to the centromeric regions. The protein relocates to the spindle midzone during late anaphase and then associates with the midbody at telophase. We have confirmed the specific pattern of immuno-localisation seen in fixed preparations by observing the distribution of Plk1 tagged with green fluorescent protein in living oocytes. We discuss the localisation of the enzyme in light of the structure of the spindle poles, which are known to lack centrioles, and the highly asymmetric nature of the meiotic divisions.
酿酒酵母两个细胞分裂周期突变的二倍体孢子形成和其他减数分裂效应。
影响因子：
3.3
作者：
Schild,D;Byers,B
通讯作者：
Byers,B
影响因子：
3.3
作者：
Cao, LG;Wang, YL
通讯作者：
Wang, YL