Effects of hepatitis E virus infection on interferon production via ISG15.
Effects of hepatitis E virus infection on interferon production via ISG15.
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戊型肝炎病毒感染对 ISG15 干扰素产生的影响
DOI：
10.3748/wjg.v24.i20.2173
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发表时间：
2018-05-28
影响因子：
4.3
通讯作者：
Tian DY
中科院分区：
文献类型：
--
作者：
Wang M;Huang Y;He M;Peng WJ;Tian DY
评价戊型肝炎病毒（HEV）对I型干扰素（IFN）产生的影响，并确定其潜在机制。 采用酶联免疫吸附试验（ELISA）检测不同时间点（0、8、12、24、48、72和120 h）基因3型HEV感染C3 A细胞中干扰素（IFN）-α和-β（-α/β）的产生。免疫印迹法检测不同时间点HEV感染C3 A细胞后IFN刺激基因（ISG）15的表达水平。将表达开放阅读框3（ORF 3）的质粒或对照质粒（表达绿色荧光蛋白）转染入C3 A细胞，并分别评估IFN-α/β和ISG 15的水平。此外，将表达ISG 15的质粒或抑制小干扰RNA的ISG 15转染到感染的C3 A细胞中。然后，还通过ELISA测量IFN-α/β的产生。结果表明，基因3型HEV能促进C3 A细胞IFN-α/β的产生，并诱导ISG 15的升高。HEV ORF 3蛋白能促进IFN-α/β的产生和ISG 15的表达。此外，ISG 15沉默增强了IFN-α/β的产生。ISG 15的过表达导致IFN-α/β的减少。HEV感染早期可通过ORF 3促进IFN-α/β的产生和ISG 15的表达，而ISG 15的增加可抑制IFN-α/β的产生。
To assess the effects of hepatitis E virus (HEV) on the production of type I interferons (IFNs) and determine the underlying mechanisms. We measured the production of interferon (IFN)-alpha and -beta (-α/β) in genotype 3 HEV-infected C3A cells at different time points (0, 8, 12, 24, 48, 72 and 120 h) by enzyme-linked immunosorbent assay (ELISA). The expression levels of IFN-stimulated gene (ISG)15 in HEV-infected C3A cells at different time points were tested by western blotting. The plasmid-expressing open reading frame 3 (ORF3) or control plasmids (green fluorescent protein-expressing) were transfected into C3A cells, and the levels of IFN-α/β and ISG15 were evaluated, respectively. Furthermore, the plasmid-expressing ISG15 or small interfering RNA-inhibiting ISG15 was transfected into infected C3A cells. Then, the production of IFN-α/β was also measured by ELISA. We showed that genotype 3 HEV could enhance the production of IFN-α/β and induce elevation of ISG15 in C3A cells. HEV ORF3 protein could enhance the production of IFN-α/β and the expression of ISG15. Additionally, ISG15 silencing enhanced the production of IFN-α/β. Overexpression of ISG15 resulted in the reduction of IFN-α/β. HEV may promote production of IFN-α/β and expression of ISG15 via ORF3 in the early stages, and increased ISG15 subsequently inhibited the production of IFN-α/β.
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影响因子：
64.5
作者：
Skaug B;Chen ZJ
通讯作者：
Chen ZJ
DOI：
10.1073/pnas.0909144107
发表时间：
2010-02-02
影响因子：
11.1
作者：
Zhao, Chen;Hsiang, Tien-Ying;Krug, Robert M.
通讯作者：
Krug, Robert M.
影响因子：
6.7
作者：
Arnaud N;Dabo S;Akazawa D;Fukasawa M;Shinkai-Ouchi F;Hugon J;Wakita T;Meurs EF
通讯作者：
Meurs EF
患有急性淋巴细胞白血病的儿童持续性戊型肝炎病毒基因型 4 感染。
影响因子：
0.6
作者：
Geng Y;Zhang H;Huang W;J Harrison T;Geng K;Li Z;Wang Y
通讯作者：
Wang Y
影响因子：
64.8
作者：
Zhang, Xianqin;Bogunovic, Dusan;Payelle-Brogard, Beatrice;Francois-Newton, Veronique;Speer, Scott D.;Yuan, Chao;Volpi, Stefano;Li, Zhi;Sanal, Ozden;Mansouri, Davood;Tezcan, Ilhan;Rice, Gillian I.;Chen, Chunyuan;Mansouri, Nahal;Mahdaviani, Seyed Alireza;Itan, Yuval;Boisson, Bertrand;Okada, Satoshi;Zeng, Lu;Wang, Xing;Jiang, Hui;Liu, Wenqiang;Han, Tiantian;Liu, Delin;Ma, Tao;Wang, Bo;Liu, Mugen;Liu, Jing-Yu;Wang, Qing K.;Yalnizoglu, Dilek;Radoshevich, Lilliana;Uze, Gilles;Gros, Philippe;Rozenberg, Flore;Zhang, Shen-Ying;Jouanguy, Emmanuelle;Bustamante, Jacinta;Garcia-Sastre, Adolfo;Abel, Laurent;Lebon, Pierre;Notarangelo, Luigi D.;Crow, Yanick J.;Boisson-Dupuis, Stephanie;Casanova, Jean-Laurent;Pellegrini, Sandra
通讯作者：
Pellegrini, Sandra