Involvement of functional protein kinase C in the mitogenic response to the H-ras oncogene product
Involvement of functional protein kinase C in the mitogenic response to the H-ras oncogene product
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功能性蛋白激酶 C 参与 H-ras 癌基因产物的有丝分裂反应
DOI：
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发表时间：
1987
影响因子：
5.3
通讯作者：
STUART A. Aaronson
中科院分区：
文献类型：
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作者：
J. Lacal;Timothy P. Fleming;Barbour S. Warren;Peter M. Blumberg;STUART A. Aaronson
将纯化的蛋白激酶 C (PKC) 显微注射到用佛波酯佛波醇 12,13-二丁酸酯预处理的瑞士 3T3 成纤维细胞中，可恢复细胞对佛波醇-12,13-二丁酸酯的有丝分裂反应（G. Pasti、J.C. Lacal、B.S. Warren、S.A. Aaronson 和 P.M. Blumberg，Nature [伦敦] 324：375-377，1986）。我们目前的研究表明，在长期佛波酯治疗下调 PKC 的条件下，显微注射 H-ras p21 的静止细胞中 H-ras 癌基因的有丝分裂活性显着降低。显微注射 H-ras p21 和 PKC 后重建促有丝分裂反应的能力意味着功能性 PKC 参与了 H-ras 癌基因产物的促有丝分裂活性。
Microinjection of purified protein kinase C (PKC) into Swiss 3T3 fibroblasts pretreated with the phorbol ester phorbol-12,13-dibutyrate restores the mitogenic response of the cells to phorbol-12,13-dibutyrate (G. Pasti, J.C. Lacal, B.S. Warren, S.A. Aaronson, and P.M. Blumberg, Nature [London] 324:375-377, 1986). Our present studies demonstrate that the mitogenic activity of the H-ras oncogene in H-ras p21-microinjected quiescent cells is markedly reduced under conditions in which PKC is downregulated by chronic phorbol ester treatment. The ability to reconstitute the mitogenic response upon microinjection of both H-ras p21 and PKC implies involvement of functional PKC in the mitogenic activity of the H-ras oncogene product.
影响因子：
56.9
作者：
MAJERUS, PW;CONNOLLY, TM;WILSON, DB
通讯作者：
WILSON, DB