Identification, characterization, and functional analysis of Tube and Pelle homologs in the mud crab Scylla paramamosain.
Identification, characterization, and functional analysis of Tube and Pelle homologs in the mud crab Scylla paramamosain.
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泥蟹scylla paramamosain中试管和pelle同源物的识别，表征和功能分析。
DOI：
10.1371/journal.pone.0076728
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Fang WH
中科院分区：
文献类型：
--
作者：
Li XC;Zhang XW;Zhou JF;Ma HY;Liu ZD;Zhu L;Yao XJ;Li LG;Fang WH
Tube 和 Pelle 是果蝇 Toll 信号通路的重要组成部分。在这项研究中，我们鉴定了拟青蟹中Tube和Pelle的一对甲壳类同源物，即SpTube和SpPelle，并分析了它们的免疫功能。 SpTube的cDNA全长2052bp，开放阅读框(ORF)长1578bp，编码525个氨基酸的蛋白质。在推导的蛋白质中预测了死亡结构域（DD）和激酶结构域。 SpPelle的全长cDNA为3825bp，ORF为3420bp，编码1140个氨基酸的蛋白质。该蛋白质含有 DD 和激酶结构域。在这两个结构域之间发现了两个保守的重复基序，以前称为管重复基序，仅存在于昆虫管或管状序列中。比对和结构预测表明 SpTubeDD 和 SpPelleDD 在序列和 3D 结构上存在显着差异。与 TubeDD 类似，SpTubeDD 在一个表面上包含三个可能介导 SpMyD88 结合的常见保守残基（R、K 和 R），在另一表面上包含两个可能有助于 Pelle 结合的常见残基（A 和 A）。相比之下，SpPelleDD 缺乏类似的保守残基。 SpTube、昆虫管样激酶和人类 IRAK4 被发现是 RD 激酶，在激酶结构域中具有 RD 二肽。 SpPelle、Pelle、昆虫 Pelle 样激酶和人类 IRAK1 被发现是缺乏 RD 二肽的非 RD 激酶。 SpTube 和 SpPelle 在血细胞、鳃和肝胰腺中高表达。攻击后，革兰氏阴性或革兰氏阳性细菌的血细胞中 SpTube 和 SpPele 显着增加，而白斑综合症病毒仅 SpPelle 升高。 Pull-down 测定表明 SpTube 可以结合 SpMyD88 和 SpPelle。这些结果表明，SpTube、SpPelle 和 SpMyD88 可能形成三聚体复合物，参与泥蟹对革兰氏阴性菌和革兰氏阳性菌的免疫。
Tube and Pelle are essential components in Drosophila Toll signaling pathway. In this study, we characterized a pair of crustacean homologs of Tube and Pelle in Scylla paramamosain, namely, SpTube and SpPelle, and analyzed their immune functions. The full-length cDNA of SpTube had 2052 bp with a 1578 bp open reading frame (ORF) encoding a protein with 525 aa. A death domain (DD) and a kinase domain were predicted in the deduced protein. The full-length cDNA of SpPelle had 3825 bp with a 3420 bp ORF encoding a protein with 1140 aa. The protein contained a DD and a kinase domain. Two conserved repeat motifs previously called Tube repeat motifs present only in insect Tube or Tube-like sequences were found between these two domains. Alignments and structure predictions demonstrated that SpTubeDD and SpPelleDD significantly differed in sequence and 3D structure. Similar to TubeDD, SpTubeDD contained three common conserved residues (R, K, and R) on one surface that may mediate SpMyD88 binding and two common residues (A and A) on the other surface that may contribute to Pelle binding. By contrast, SpPelleDD lacked similar conservative residues. SpTube, insect Tube-like kinases, and human IRAK4 were found to be RD kinases with an RD dipeptide in the kinase domain. SpPelle, Pelle, insect Pelle-like kinases, and human IRAK1 were found to be non-RD kinases lacking an RD dipeptide. Both SpTube and SpPelle were highly expressed in hemocytes, gills, and hepatopancreas. Upon challenge, SpTube and SpPele were significantly increased in hemocytes by Gram-negative or Gram-positive bacteria, whereas only SpPelle was elevated by White Spot Syndrome Virus. The pull-down assay showed that SpTube can bind to both SpMyD88 and SpPelle. These results suggest that SpTube, SpPelle, and SpMyD88 may form a trimeric complex involved in the immunity of mud crabs against both Gram-negative and Gram-positive bacteria.
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Tube 是 Toll 通路中的 IRAK-4 同源物，适合发育和免疫。
影响因子：
5.3
作者：
Towb, Par;Sun, Huaiyu;Wasserman, Steven A.
通讯作者：
Wasserman, Steven A.
影响因子：
2.9
作者：
Huang, Xian-De;Yin, Zhi-Xin;He, Jian-Guo
通讯作者：
He, Jian-Guo
影响因子：
4.7
作者：
Arts, Joop A. J.;Cornelissen, Ferry H. J.;Stet, Rene J. M.
通讯作者：
Stet, Rene J. M.
DOI：
10.1073/pnas.202396399
发表时间：
2002-10-01
影响因子：
11.1
作者：
Sun, HY;Bristow, BN;Wasserman, SA
通讯作者：
Wasserman, SA
影响因子：
14.8
作者：
Kelley, Lawrence A.;Sternberg, Michael J. E.
通讯作者：
Sternberg, Michael J. E.