Fork head transcription factor is required for ovarian mature in the brown planthopper, Nilaparvata lugens (Stål).
Fork head transcription factor is required for ovarian mature in the brown planthopper, Nilaparvata lugens (Stål).
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DOI：
10.1186/1471-2199-12-53
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发表时间：
2011-12-31
影响因子：
--
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Dong X;Zhai Y;Zhang J;Sun Z;Chen J;Chen J;Zhang W
褐飞虱（Nilaparvata lugens）是亚洲许多地区最具破坏性的水稻害虫。雌虫的生殖系统。褐飞虱由一对子房组成，大多数个体每个子房有24-33个卵巢管，这决定了其繁殖力。叉头（Fox）是一种转录调控分子，在真核生物中调节和控制许多生理过程。福克斯家族有几个子类和成员，据报道，几个福克斯因子参与调节繁殖力。我们在N. lugens。NlFoxA全长cDNA为1789 bp，开放阅读框为1143 bp，编码380个氨基酸的蛋白质。实时定量PCR（RT-qPCR）和逆转录PCR（RT-PCR）分析表明，NlFoxA mRNA主要在脂肪体、中肠、表皮和马氏管中表达，且在整个发育过程中持续表达，变化不大。NlFoxA属于Fox转录因子的FoxA亚家族。使用含有双链RNA（dsRNA）的人工饲料通过RNAi敲低NlFoxA表达显著减少了后代的数量并影响卵巢的发育。ELISA和Westernblot分析表明，NlFoxA基因的取食型RNAi也导致卵黄蛋白原（Vg）蛋白表达量下降。NlFoxA通过调控卵黄蛋白原的表达，在BPH的生殖和卵巢发育中起重要作用。
The brown planthopper (BPH), Nilaparvata lugens, is the most devastating rice pest in many areas throughout Asia. The reproductive system of female N. lugens consists of a pair of ovaries with 24-33 ovarioles per ovary in most individuals which determine its fecundity. The fork head (Fox) is a transcriptional regulatory molecule, which regulates and controls many physiological processes in eukaryotes. The Fox family has several subclasses and members, and several Fox factors have been reported to be involved in regulating fecundity. We have cloned a fork head gene in N. lugens. The full-length cDNA of NlFoxA is 1789 bp and has an open reading frame of 1143 bp, encoding a protein of 380 amino acids. Quantitative real-time PCR (RT-qPCR) and Reverse Transcription- PCR (RT-PCR) analysis revealed that NlFoxA mRNA was mainly expressed in the fat body, midgut, cuticle and Malpighian tube, and was expressed continuously with little change during all the developmental stages. NlFoxA belongs to the FoxA subfamily of the Fox transcription factors. Knockdown of NlFoxA expression by RNAi using artificial diet containing double-stranded RNA (dsRNA) significantly decreased the number of offspring and impacted the development of ovaries. ELISA and Western blot analyses showed that feeding-based RNAi of NlFoxA gene also resulted in decreased expression of vitellogenin (Vg) protein. NlFoxA plays an important role in regulation of fecundity and development of ovaries in the BPH via regulating vitellogenin expression.
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影响因子：
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作者：
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影响因子：
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