Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury.
Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury.
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DOI：
10.3969/j.issn.1673-5374.2013.36.001
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发表时间：
2013-12-25
影响因子：
6.1
通讯作者：
Golmohammadi MG
中科院分区：
文献类型：
--
作者：
Najafzadeh N;Nobakht M;Pourheydar B;Golmohammadi MG
利用成体干细胞治疗脊髓损伤 (SCI) 的新兴研究使我们能够评估毛囊干细胞移植对患有压迫性脊髓损伤的大鼠的效果。在这里，我们提出了一个假设：大鼠毛囊干细胞移植可以促进受损脊髓的恢复。本研究在 Wistar 大鼠中诱发压迫性脊髓损伤。分离、培养大鼠触须毛囊的隆起区域，并用巢蛋白作为干细胞标记物进行表征。将5-溴-2'-脱氧尿苷（BrdU）标记的凸出干细胞移植到脊髓损伤的大鼠体内。免疫组织化学染色结果显示，移植后3周，部分移植细胞能够存活并分化为少突胶质细胞（受体相互作用蛋白阳性细胞）和神经元样细胞（βIII-微管蛋白阳性细胞）。此外，使用 Basso、Beattie 和 Bresnahan (BBB) 运动评定量表评估细胞移植后 8 周时脊髓损伤大鼠后肢运动功能的恢复情况。结果表明，移植的毛囊干细胞可以在体内长期存活并分化为神经元和胶质细胞样细胞。这些结果表明毛囊干细胞可以促进脊髓损伤的恢复。
Emerging studies of treating spinal cord injury (SCI) with adult stem cells led us to evaluate the effects of transplantation of hair follicle stem cells in rats with a compression-induced spinal cord lesion. Here, we proposed a hypothesis that rat hair follicle stem cell transplantation can promote the recovery of injured spinal cord. Compression-induced spinal cord injury was induced in Wistar rats in this study. The bulge area of the rat vibrissa follicles was isolated, cultivated and characterized with nestin as a stem cell marker. 5-Bromo-2′-deoxyuridine (BrdU) labeled bulge stem cells were transplanted into rats with spinal cord injury. Immunohistochemical staining results showed that some of the grafted cells could survive and differentiate into oligodendrocytes (receptor-interacting protein positive cells) and neuronal-like cells (βIII-tubulin positive cells) at 3 weeks after transplantation. In addition, recovery of hind limb locomotor function in spinal cord injury rats at 8 weeks following cell transplantation was assessed using the Basso, Beattie and Bresnahan (BBB) locomotor rating scale. The results demonstrate that the grafted hair follicle stem cells can survive for a long time period in vivo and differentiate into neuronal- and glial-like cells. These results suggest that hair follicle stem cells can promote the recovery of spinal cord injury.
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影响因子：
64.5
作者：
Hsu YC;Pasolli HA;Fuchs E
通讯作者：
Fuchs E
DOI：
10.1073/pnas.0501263102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Amoh, Y;Li, L;Hoffman, RM
通讯作者：
Hoffman, RM
影响因子：
2.5
作者：
HOULE, JD;REIER, PJ
通讯作者：
REIER, PJ
影响因子：
4.8
作者：
Hu, Yao Fei;Gourab, Krishnaj;Wells, Clive;Clewes, Oliver;Schmit, Brian D.;Sieber-Blum, Maya
通讯作者：
Sieber-Blum, Maya
影响因子：
3.4
作者：
Huang, W. L.;George, K. J.;Priestley, J. V.
通讯作者：
Priestley, J. V.