Dynamics between stem cells, niche, and progeny in the hair follicle.
Dynamics between stem cells, niche, and progeny in the hair follicle.
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DOI：
10.1016/j.cell.2010.11.049
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发表时间：
2011-01-07
期刊：
影响因子：
64.5
通讯作者：
Fuchs E
中科院分区：
文献类型：
--
作者：
Hsu YC;Pasolli HA;Fuchs E
在这里，我们利用毛囊来定义干细胞沿着分化谱系不可逆地沿着定型的点。采用组蛋白和核苷酸双脉冲追踪和谱系追踪，我们发现，早期SC后裔途中成为过境扩增细胞保留干细胞和慢循环特性，并在头发生长停止时回到凸起的生态位。这些成为下一个毛发周期的主要SC，而最初的隆起SC成为损伤的储备。在进一步的过程中，扩增的后代不可逆地失去了它们的干性，尽管它们保留了许多SC标记并存活下来，不像它们的过渡扩增后代。值得注意的是，这些后代也回到了隆起的家。我们将纯化和基因表达分析与差异消融和功能实验相结合，定义了这些非SC生态位居民的关键功能，并揭示了一个有趣的概念，即SC谱系中的不可逆定型细胞可以成为生态位微环境的重要贡献者。
Here, we exploit the hair follicle to define the point at which stem cells become irreversibly committed along a differentiation lineage. Employing histone and nucleotide double-pulse-chase and lineage tracing, we show that the early SC descendents en route to becoming transit-amplifying cells retain stemness and slow-cycling properties and home back to the bulge niche when hair growth stops. These become the primary SCs for the next hair cycle, while initial bulge SCs become reserves for injury. Proliferating descendents further en route irreversibly lose their stemness, although they retain many SC markers and survive, unlike their transit-amplifying progeny. Remarkably, these progeny also home back to the bulge. Combining purification and gene expression analysis with differential ablation and functional experiments, we define critical functions for these non-SC niche residents, and unveil the intriguing concept that an irreversibly committed cell in an SC lineage can become an essential contributor to the niche microenvironment.
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