Pure pressure stress increased monocarboxylate transporter in human aortic smooth muscle cell membrane
Pure pressure stress increased monocarboxylate transporter in human aortic smooth muscle cell membrane
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纯压力应激增加人主动脉平滑肌细胞膜中的单羧酸转运蛋白
DOI：
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发表时间：
2004
影响因子：
4.3
通讯作者：
H. Kawaguchi
中科院分区：
文献类型：
--
作者：
Koji Oikawa;K. Iizuka;T. Murakami;T. Nagai;K. Okita;K. Yonezawa;A. Kitabatake;H. Kawaguchi
乳酸在充分有氧条件下不断形成和利用。乳酸在任何时候都会被积极氧化，尤其是在运动期间。在细胞和组织中差异表达的单羧酸转运蛋白 (MCT) 家族可促进乳酸跨膜转运。之前我们报道过血液循环中存在MCT1。我们还报告了压力刺激刺激主动脉平滑肌细胞（HASMC）的细胞增殖。在本实验中，我们试图证明HASMC中MCT1的存在，并阐明压力应力对HASMC中MCT1定位的影响。我们确定琥珀酸脱氢酶（SDH）活性作为细胞能量代谢的标志。 SDH 活性因压力而增加。乳酸以剂量依赖性方式增强压力应激（160 mmHg，3 小时）下的 SDH 活性。另一方面，添加乳酸抑制了乳酸排泄。我们可以在 HASMC 的胞质和膜部分中检测到 MCT1。在细胞外乳酸存在的情况下，压力应激增加了膜部分中的 MCT1。综上，我们证明了HASMC中MCT1的存在。压力改变了MCT1的定位。增加的膜MCT1可以运输乳酸用于细胞内的能量代谢。
Lactate is formed and utilized continuously under fully aerobic conditions. Lactate is oxidized actively at all times, especially during exercise. Family of monocarboxylate transport proteins (MCTs) that are differentially expressed in cells and tissues accomplishes the facilitated transport of lactate across membranes. Previously we reported that there is MCT1 in blood circulation. We also reported the pressure stress stimulated cell proliferation in aortic smooth muscle cells (HASMC). In this experiment we attempted to prove the existence of MCT1 in HASMC and to clarify the effect of pressure stress on MCT1 localization in HASMC. We determined succinate dehydrogenase (SDH) activity as a marker of energy metabolism in cells. SDH activity was increased by pressure stress. Lactate enhanced the SDH activity under pressure stress (160 mmHg for 3 h) as dose dependent manner. On the other hand, lactate excretion was suppressed by the addition of lactate. We could detect MCT1 in the cytosolic and the membrane fractions of HASMC. The pressure stress increased MCT1 in the membrane fraction in the presence of extracellular lactate. In summary, we proved the existence of MCT1 in HASMC. Pressure stress changed the localization of MCT1. The increased membranous MCT1 may transport lactate for energy metabolism in cells.
DOI：
10.1016/s0305-0491(98)00025-x
发表时间：
1998-05-01
影响因子：
2.2
作者：
Brooks, GA
通讯作者：
Brooks, GA
静态张力与大鼠肺动脉中平滑肌细胞 DNA 合成的增加有关。
DOI：
10.1152/ajpheart.1995.268.3.h1133
发表时间：
1995
期刊：
The American journal of physiology
影响因子：
--
作者：
Weiser,MC;Majack,RA;Tucker,A;Orton,EC
通讯作者：
Orton,EC
DOI：
10.1073/pnas.96.3.1129
发表时间：
1999-02-02
影响因子：
11.1
作者：
Brooks, GA;Dubouchaud, H;Butz, CE
通讯作者：
Butz, CE